Holland K T, Taylor D, Farrell A M
Microbiology Department, University of Leeds, UK.
J Antimicrob Chemother. 1994 Jan;33(1):41-55. doi: 10.1093/jac/33.1.41.
The effects of glycerol monolaurate on the growth of, and production of Toxic Shock Syndrome Toxin-1 (TSST-1) and lipase by, Staphylococcus aureus FRI 1187 were investigated in batch and continuous culture models using a defined synthetic medium. The growth and yield of S. aureus depended on glycerol monolaurate concentration, pH of the environment and the inoculum size. The MICs for glycerol monolaurate of 29 S. aureus isolates, determined in a complex medium, were between 10 and 20 mg/L with a 10(3) to 10(4) cfu inoculum; a 1000-fold increase in inoculum size increased the MIC five-fold. In continuous culture, glycerol monolaurate increased the cell yield of S. aureus. In batch cultures with 17 mg/L glycerol monolaurate, TSST-1 and lipase production by S. aureus was delayed and their specific production was not decreased compared with cultures without glycerol monolaurate. At 150 mg/L glycerol monolaurate, growth and toxin production were decreased. The lipase of S. aureus hydrolyzed glycerol monolaurate. The reduction of toxin production was associated with effects on S. aureus growth, and these effects might have been relieved after lipase had been produced.
在分批培养和连续培养模型中，使用特定的合成培养基，研究了单月桂酸甘油酯对金黄色葡萄球菌FRI 1187生长以及产生中毒性休克综合征毒素-1（TSST-1）和脂肪酶的影响。金黄色葡萄球菌的生长和产量取决于单月桂酸甘油酯浓度、环境pH值和接种量。在复合培养基中测定的29株金黄色葡萄球菌分离株对单月桂酸甘油酯的最低抑菌浓度（MIC）在10至20 mg/L之间，接种量为10³至10⁴ cfu；接种量增加1000倍会使MIC增加五倍。在连续培养中，单月桂酸甘油酯提高了金黄色葡萄球菌的细胞产量。在含有17 mg/L单月桂酸甘油酯的分批培养中，金黄色葡萄球菌产生TSST-1和脂肪酶的时间延迟，与不含单月桂酸甘油酯的培养相比，其比产量并未降低。在单月桂酸甘油酯浓度为150 mg/L时，生长和毒素产生均减少。金黄色葡萄球菌的脂肪酶可水解单月桂酸甘油酯。毒素产生的减少与对金黄色葡萄球菌生长的影响有关，而这些影响可能在脂肪酶产生后得到缓解。
