Institute Pasteur-Fondazione Cenci Bolognetti, Department of Clinical and Molecular Medicine, Sapienza University of Roma, Roma, Italy.
PLoS One. 2013;8(2):e57659. doi: 10.1371/journal.pone.0057659. Epub 2013 Feb 25.
The role of the mesothelial layer in the peritoneal spreading of cancer cells is only partially clarified. Here we attempted to better define the mesothelial contribution to the tumor cell adhesion using a direct adhesion test applied to human primary cultures of mesothelial cells (HPMCs) derived from the peritoneal washes of patients with gastric and colorectal cancers. Gastric and colon carcinoma cells were seeded on different mesothelial monolayers and quantitative fluorescence analysis was performed to analyze their growth and adhesive properties. The adhesion of the cancer cells was not affected by the origin of the HPMCs when derived from patients with different cancers or with benign disease. In contrast, the high levels of ICAM1 expression and ROS production, which characterize these senescent mesothelial cells, enhanced the tumor cell adhesion. These results suggest that the mesothelial adhesive properties are dependent on the cell senescence, while are not affected by the tumor environment. The use of peritoneal washes as a source to isolate HPMCs provides a practical and reliable tool for the in vitro analysis of the mesothelial conditions affecting the peritoneal carcinomatosis.
间皮细胞层在癌细胞腹膜扩散中的作用尚未完全阐明。在这里，我们试图使用直接粘附试验，对源自胃癌和结直肠癌患者腹腔冲洗液的人原代间皮细胞（HPMC），更好地定义间皮细胞对肿瘤细胞粘附的贡献。将胃癌和结肠癌细胞接种在不同的间皮单层上，并进行定量荧光分析，以分析它们的生长和粘附特性。当源自不同癌症或良性疾病患者时，HPMC 的来源并不影响癌细胞的粘附。相比之下，这些衰老的间皮细胞特征性的高水平 ICAM1 表达和 ROS 产生，增强了肿瘤细胞的粘附。这些结果表明，间皮细胞的粘附特性取决于细胞衰老，而不受肿瘤环境的影响。使用腹腔冲洗液作为分离 HPMC 的来源，为体外分析影响腹膜癌转移的间皮状态提供了一种实用且可靠的工具。
