Raabe T, Schelle-Prinz B, Siddell S G
Institute of Virology, University of Würzburg, F.R.G.
J Gen Virol. 1990 May;71 ( Pt 5):1065-73. doi: 10.1099/0022-1317-71-5-1065.
The gene encoding the spike glycoprotein of the human coronavirus HCV 229E has been cloned and sequenced. This analysis predicts an S polypeptide of 1173 amino acids with an Mr of 128,600. The polypeptide has 30 potential N-glycosylation sites. A number of structural features typical of coronavirus S proteins can be recognized, including a signal sequence, a membrane anchor, heptad repeat structures and a carboxy-terminal cysteine cluster. A detailed, computer-aided comparison with the S proteins of infectious bronchitis virus, feline infectious peritonitis virus, transmissible gastroenteritis virus and murine hepatitis virus, strain JHM is presented. We have also done a Northern blot analysis of viral RNAs in HCV 229E-infected cells using synthetic oligonucleotides. On the basis of this analysis, and by analogy to the replication strategy of other coronaviruses, we are able to propose a model for the organization and expression of the HCV 229E genome.
编码人冠状病毒HCV 229E刺突糖蛋白的基因已被克隆和测序。该分析预测出一个含有1173个氨基酸、分子量为128,600的S多肽。该多肽有30个潜在的N-糖基化位点。可以识别出许多典型的冠状病毒S蛋白的结构特征，包括信号序列、膜锚定、七肽重复结构和羧基末端半胱氨酸簇。本文对传染性支气管炎病毒、猫传染性腹膜炎病毒、传染性胃肠炎病毒和鼠肝炎病毒JHM株的S蛋白进行了详细的计算机辅助比较。我们还使用合成寡核苷酸对HCV 229E感染细胞中的病毒RNA进行了Northern印迹分析。基于该分析，并类比其他冠状病毒的复制策略，我们能够提出一个HCV 229E基因组的组织和表达模型。
