Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
Bioresour Technol. 2013 Apr;133:606-11. doi: 10.1016/j.biortech.2013.02.016. Epub 2013 Feb 10.
Geobacter sulfurreducens was studied for the reduction of Pd(II) and production of Pd(0) nanoparticles capable of reducing Cr(VI). Transmission electronic microscopy, energy dispersive X-ray and X-ray diffraction analyses revealed that the nanoscale Pd(0) particles formed were associated with the cell surface and located inside the periplasm. The increase of cell dry weight (CDW):Pd ratio and addition of anthraquinone-2,6-disulfonate (AQDS) not only stimulated Pd(II) reduction, but also resulted in increase of nanoparticle number, decrease of particle diameter and improvement of Cr(VI) reduction efficiency. The relationship between reduction rate and initial Cr(VI) concentration (150-750 μM) followed Michaelis-Menten kinetics (Vmax=3.6 μmol h(-1) mg bio-Pd(-1) and Km=891.3 μM). These findings indicated the potential of using G. sulfurreducens cells for reclamation of palladium, formation of Pd(0) nanoparticles and efficient treatment of Cr(VI) pollution.
研究了 Geobacter sulfurreducens 对 Pd(II)的还原作用，以及生成能够还原 Cr(VI)的 Pd(0)纳米颗粒。透射电子显微镜、能量色散 X 射线和 X 射线衍射分析表明，形成的纳米级 Pd(0)颗粒与细胞表面有关，并位于周质内。细胞干重（CDW）与 Pd 的比例增加和蒽醌-2,6-二磺酸钠（AQDS）的添加不仅刺激了 Pd(II)的还原，而且还增加了纳米颗粒的数量，降低了颗粒直径，并提高了 Cr(VI)的还原效率。还原速率与初始 Cr(VI)浓度（150-750 μM）之间的关系遵循米氏动力学（Vmax=3.6 μmol h(-1) mg 生物 Pd(-1)和 Km=891.3 μM）。这些发现表明，利用 G. sulfurreducens 细胞回收钯、形成 Pd(0)纳米颗粒和有效处理 Cr(VI)污染具有潜力。
