School of Advanced Materials Science & Engineering, Sungkyunkwan University, 300 Chunchun-dong, Suwon, Gyeonggi-do 440-746, South Korea.
Biosens Bioelectron. 2013 Jul 15;45:70-6. doi: 10.1016/j.bios.2013.01.051. Epub 2013 Feb 4.
Solution-gated reduced graphene oxide field-effect transistors (R-GO FETs) were investigated for pH sensing and biochemical sensing applications. A channel of a networked R-GO film formed by self-assembly was incorporated as a sensing layer into a solution-gated FET structure for pH sensing and the detection of acetylcholine (Ach), which is a neurotransmitter in the nerve system, through enzymatic reactions. The fabricated R-GO FET was sensitive to protons (H(+)) with a pH sensitivity of 29 mV/pH in terms of the shift of the charge neutrality point (CNP), which is attributed to changes in the surface potential caused by the interaction of protons with OH surface functional groups present on the R-GO surface. The R-GO FET immobilized with acetylcholinesterase (AchE) was used to detect Ach in the concentration range of 0.1-10mM by sensing protons generated during the enzymatic reactions. The results indicate that R-GO FETs provide the capability to detect protons, demonstrating their applicability as a biosensing device for enzymatic reactions.
用于 pH 传感和生化传感应用的溶液门控还原氧化石墨烯场效应晶体管 (R-GO FET) 得到了研究。通过自组装形成的网络 R-GO 薄膜的通道被并入溶液门控 FET 结构中，用于通过酶反应进行 pH 传感和检测神经递质乙酰胆碱 (Ach)。所制造的 R-GO FET 对质子 (H(+)) 敏感，在电荷中性点 (CNP) 偏移方面，pH 灵敏度为 29 mV/pH，这归因于与 R-GO 表面上存在的 OH 表面官能团相互作用引起的表面电势变化。用乙酰胆碱酯酶 (AchE) 固定的 R-GO FET 用于通过感测在酶反应过程中产生的质子来检测 0.1-10mM 浓度范围内的 Ach。结果表明，R-GO FET 提供了检测质子的能力，证明了它们作为用于酶反应的生物传感装置的适用性。
