Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Bioresour Technol. 2013 Apr;133:482-94. doi: 10.1016/j.biortech.2013.01.126. Epub 2013 Jan 31.
Two low structured mathematical models for fed-batch production of polyhydroxybutyrate and poly[hydroxybutyrate-co-hydroxyvalerate] by Cupriavidus necator DSM 545 on renewable substrates (glycerol and fatty acid methyl esters-FAME) combined with glucose and valeric acid, were established. The models were used for development/optimization of feeding strategies of carbon and nitrogen sources concerning PHA content and polymer/copolymer composition. Glycerol/glucose fermentation featured a max. specific growth rate of 0.171 h(-1), a max. specific production rate of 0.038 h(-1) and a PHB content of 64.5%, whereas the FAME/valeric acid fermentation resulted in a max. specific growth rate of 0.046 h(-1), a max. specific production rate of 0.07 h(-1) and 63.6% PHBV content with 4.3% of 3-hydroxyvalerate (3HV) in PHBV. A strong inhibition of glycerol consumption by glucose was confirmed (inhibition constant ki,G=4.28×10(-4) g L(-1)). Applied concentration of FAME (10-12 g L(-1)) positively influenced on PHBV synthesis. HV/PHBV ratio depends on applied VA concentration.
建立了两种低结构数学模型，用于在可再生基质（甘油和脂肪酸甲酯-FAME）上生产聚羟基丁酸酯和聚[羟基丁酸酯-共-羟基戊酸酯]的分批补料生产，使用 Cupriavidus necator DSM 545。模型用于开发/优化有关 PHB 含量和聚合物/共聚物组成的碳和氮源的进料策略。甘油/葡萄糖发酵的最大比生长速率为 0.171 h(-1)，最大比生产速率为 0.038 h(-1)，PHB 含量为 64.5%，而 FAME/戊酸发酵的最大比生长速率为 0.046 h(-1)，最大比生产速率为 0.07 h(-1)，PHBV 中的 3-羟基戊酸 (3HV) 含量为 63.6%，为 4.3%。证实了葡萄糖对甘油消耗的强烈抑制作用（抑制常数 ki，G=4.28×10(-4) g L(-1)）。应用的 FAME 浓度（10-12 g L(-1)）对 PHBV 合成有积极影响。HV/PHBV 比取决于应用的 VA 浓度。
