Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Kyushu, Japan,
Ann Surg Oncol. 2013 Dec;20 Suppl 3:S509-16. doi: 10.1245/s10434-013-2914-9. Epub 2013 Mar 1.
The clinical significance of Brachyury expression and its relationship to epithelial-mesenchymal transition in primary lung carcinoma is unclear.
Expression of Brachyury mRNA was investigated in 104 surgically resected primary lung carcinoma tissues. Immunohistochemical analysis of Brachyury transcription factor, Slug, E-cadherin, IL-8, N-cadherin, and Ki67 was performed in 67 of 104 cases, and their expression was correlated to prognoses and clinicopathological factors.
Brachyury mRNA expression in primary lung carcinoma tissues was a significant predictor of poor prognosis for 5-year disease-free survival and overall survival rates and was significantly correlated to vascular invasion, lymphatic permeation, histological grade, pathologic T stage, and pathologic N stage (P < 0.05). Brachyury mRNA expression was significantly inversely correlated to E-cadherin expression (P = 0.0252) and positively correlated to IL-8 protein (P = 0.0241) and to Slug protein (P = 0.0243) in adenocarcinoma tissues.
A positive association between Brachyury and Slug and IL-8, and a negative association with E-cadherin may lead to invasiveness and metastasis in primary lung carcinoma. Brachyury mRNA expression is a significant predictor of poor prognosis in primary lung carcinoma.
Brachyury 表达的临床意义及其与原发性肺癌上皮-间充质转化的关系尚不清楚。
研究了 104 例手术切除的原发性肺癌组织中 Brachyury mRNA 的表达。对 67 例中的 Brachyury 转录因子、Slug、E-钙黏蛋白、IL-8、N-钙黏蛋白和 Ki67 进行了免疫组织化学分析，并将其表达与预后和临床病理因素相关联。
原发性肺癌组织中 Brachyury mRNA 的表达是 5 年无病生存率和总生存率的不良预后的显著预测因子，与血管侵犯、淋巴渗透、组织学分级、病理 T 分期和病理 N 分期显著相关（P < 0.05）。Brachyury mRNA 的表达与 E-钙黏蛋白的表达呈显著负相关（P = 0.0252），与腺癌组织中的 IL-8 蛋白（P = 0.0241）和 Slug 蛋白（P = 0.0243）呈显著正相关。
Brachyury 与 Slug 和 IL-8 的正相关，与 E-钙黏蛋白的负相关可能导致原发性肺癌的侵袭和转移。Brachyury mRNA 的表达是原发性肺癌不良预后的显著预测因子。
