The Helmholtz Sino-German Research Laboratory for Cancer, Department of Pathology, Tangdu Hospital, Fourth Military Medical University, Xi'an, People's Republic of China.
Exp Mol Pathol. 2013 Dec;95(3):270-5. doi: 10.1016/j.yexmp.2013.07.008. Epub 2013 Jul 29.
PTPN13 is a new candidate tumor-suppressing gene. To investigate the PTPN13 expression and its potential function in the invasion and metastasis of lung squamous cell carcinoma (LSCC), we performed this study in 91 primary LSCC tissues and the adjacent non-cancerous tissues.
The mRNA expression of PTPN13 and FAK was quantitated by reverse transcription polymerase chain reaction. The protein expression of PTPN13, focal adhesion kinase (FAK) and phosphorylated FAK (P-FAK) was evaluated using immunohistochemical staining and western blotting. The association among PTPN13 expression, FAK expression and the clinicopathological parameters were analyzed.
PTPN13 expression was down-regulated in LSCC, and was negatively correlated with the cancer grade and stage. FAK mRNA, as well as FAK protein level was elevated in LSCC tissues. P-FAK level, also found increased, had no association with FAK mRNA and FAK protein expression, but had a negative correlation with the PTPN13 expression. P-FAK level had a significant positive correlation with the TNM classification.
The over-expression of FAK and increased FAK phosphorylation plays an important role in the invasion and metastasis of LSCC.
PTPN13 是一种新的候选抑癌基因。为了研究 PTPN13 在肺鳞癌（LSCC）侵袭和转移中的表达及其潜在功能，我们在 91 例原发性 LSCC 组织及其相邻非癌组织中进行了这项研究。
采用逆转录聚合酶链反应定量检测 PTPN13 和 FAK 的 mRNA 表达。采用免疫组织化学染色和 Western blot 检测 PTPN13、黏着斑激酶（FAK）和磷酸化 FAK（P-FAK）的蛋白表达。分析 PTPN13 表达、FAK 表达与临床病理参数之间的相关性。
PTPN13 在 LSCC 中表达下调，与癌症分级和分期呈负相关。LSCC 组织中 FAK mRNA 以及 FAK 蛋白水平升高。P-FAK 水平也升高，与 FAK mRNA 和 FAK 蛋白表达无相关性，但与 PTPN13 表达呈负相关。P-FAK 水平与 TNM 分级有显著的正相关性。
FAK 的过度表达和 FAK 磷酸化的增加在 LSCC 的侵袭和转移中发挥重要作用。
