Department of Chemistry, University of Massachusetts Lowell, Lowell, Massachusetts 01842, USA.
Biomacromolecules. 2013 Apr 8;14(4):1054-62. doi: 10.1021/bm301922z. Epub 2013 Mar 20.
Cellulose-ethylenediaminetetraacetic acid (EDTA) conjugates were synthesized by the esterification of cellulose with ethylenediaminetetraacetic dianhydride (EDTAD). The new materials provided potent antimicrobial activities against Staphylococcus aureus (S. aureus, Gram-positive bacteria) and Pseudomonas aeruginosa (P. aeruginosa, Gram-negative bacteria), and inhibited the formation of bacterial biofilms. The biocompatibility of the new cellulose-EDTA conjugates was evaluated with mouse skin fibroblasts for up to 14 days. SEM observation and DNA content analysis suggested that the new materials sustained the viability of fibroblast cells. Moreover, in mouse skin fibroblast-bacteria co-culture systems, the new cellulose-EDTA conjugates prevented bacterial biofilm formation and protected the mammalian cells from the bacterial cells for at least one day.
纤维素-乙二胺四乙酸（EDTA）缀合物是通过纤维素与乙二胺四乙酸二酐（EDTAD）的酯化反应合成的。这些新材料对金黄色葡萄球菌（革兰氏阳性菌）和铜绿假单胞菌（革兰氏阴性菌）具有很强的抗菌活性，并抑制了细菌生物膜的形成。新的纤维素-EDTA 缀合物的生物相容性通过长达 14 天的小鼠皮肤成纤维细胞进行评估。SEM 观察和 DNA 含量分析表明，新材料维持了成纤维细胞的活力。此外，在小鼠皮肤成纤维细胞-细菌共培养系统中，新的纤维素-EDTA 缀合物可以防止细菌生物膜的形成，并保护哺乳动物细胞免受细菌细胞的侵害，至少持续一天。
