Drago Lorenzo, De Vecchi Elena, Mattina Roberto, Romanò Carlo L
Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopedic Institute, 20161 Milan, Italy.
Int J Artif Organs. 2013 Jan;36(1):39-46. doi: 10.5301/ijao.5000135.
Staphylococcus aureus and Pseudomonas aeruginosa are frequently isolated from biofilm-associated orthopedic implant infections. This study assessed the activity of N-acetyl-L-cysteine at inhibitory and subinhibitory concentrations against biofilm formed by S. aureus and P. aeruginosa on prosthetic materials.
S. aureus and P. aeruginosa biofilm on smooth polyethylene and sand-blasted titanium discs was determined by means of a spectrophotometric assay before and at 3, 6, and 18 hours after addition of N-acetyl-L-cysteine.
N-acetyl-L-cysteine was able to disaggregate biofilm produced by S. aureus and P. aeruginosa on polyethylene and titanium discs after 3 h of incubation. A different rate in eradication of biofilm produced by P. aeruginosa was seen between polyethylene and titanium (50% vs. 20% respectively vs. biofilm formed before treatment). Results were confirmed by confocal laser scanning microscopy.
The results suggest a possible role of N-acetyl-L-cysteine in the treatment of orthopedic prosthetic infections.
金黄色葡萄球菌和铜绿假单胞菌经常从与生物膜相关的骨科植入物感染中分离出来。本研究评估了N-乙酰-L-半胱氨酸在抑制浓度和亚抑制浓度下对金黄色葡萄球菌和铜绿假单胞菌在假体材料上形成的生物膜的活性。
通过分光光度法在添加N-乙酰-L-半胱氨酸之前以及添加后3、6和18小时测定光滑聚乙烯和喷砂钛盘上的金黄色葡萄球菌和铜绿假单胞菌生物膜。
孵育3小时后，N-乙酰-L-半胱氨酸能够分解金黄色葡萄球菌和铜绿假单胞菌在聚乙烯和钛盘上产生的生物膜。在聚乙烯和钛之间观察到铜绿假单胞菌产生的生物膜的清除率不同（分别为50%和20%，与处理前形成的生物膜相比）。共聚焦激光扫描显微镜证实了结果。
结果表明N-乙酰-L-半胱氨酸在骨科假体感染治疗中可能发挥作用。
