State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Shenzhen, Guangdong, PR China.
J Food Sci. 2013 Mar;78(3):M452-7. doi: 10.1111/1750-3841.12051.
The ingredient declaration on food labels assumes paramount importance in the protection of food-allergic consumers. China has not implemented Food allergen labeling. A gold immunochromatography assay (GICA) was developed using 2 monoclonal antibodies (mAb) against the milk allergen β-lactoglobulin in this study. The GICA was specific for pure milk samples with a sensitivity of 0.2 ng/mL. Milk protein traces extracted from 110 food products were detected by this method. The labels of 106 were confirmed by our GICA method: 57 food samples originally labeled as containing milk were positive for β-lactoglobulin and 49 food samples labeled as not containing milk were negative for β-lactoglobulin. However, 3 food samples falsely labeled as containing milk were found to contain no β-lactoglobulin whereas 1 food sample labeled as not containing milk actually contained β-lactoglobulin. First, these negatives could be because of the addition of a casein fraction. Second, some countries demand that food manufacturers label all ingredients derived from milk as "containing milk" even though the ingredients contain no detectable milk protein by any method. Our GICA method could thus provide a fast and simple method for semiquantitatation of β-lactoglobulin in foods.
The present method provides a fast, simple, semiquantitative method for the determination of milk allergens in foods.
食品标签上的成分声明在保护食物过敏消费者方面至关重要。中国尚未实施食物过敏原标签。本研究使用针对牛奶过敏原β-乳球蛋白的 2 种单克隆抗体（mAb）开发了一种金免疫层析测定法（GICA）。该 GICA 对纯牛奶样品具有特异性，灵敏度为 0.2ng/mL。通过该方法检测了从 110 种食品中提取的牛奶蛋白痕迹。我们的 GICA 方法证实了 106 个标签：57 种最初标记为含牛奶的食品样品β-乳球蛋白检测结果呈阳性，49 种标记为不含牛奶的食品样品β-乳球蛋白检测结果呈阴性。然而，发现 3 种被错误标记为含牛奶的食品实际上不含β-乳球蛋白，而 1 种被标记为不含牛奶的食品实际上含有β-乳球蛋白。首先，这些阴性结果可能是由于添加了酪蛋白部分。其次，一些国家要求食品制造商将所有源自牛奶的成分标记为“含牛奶”，即使任何方法都无法检测到这些成分中的牛奶蛋白。因此，我们的 GICA 方法可以为食品中β-乳球蛋白的半定量提供一种快速简单的方法。
本方法提供了一种快速、简单、半定量的食品中牛奶过敏原检测方法。
