一种用于快速检测中国进出口食品中花生过敏原Ara h1的双位点单克隆抗体免疫层析检测方法。
A two-site monoclonal antibody immunochromatography assay for rapid detection of peanut allergen Ara h1 in Chinese imported and exported foods.
作者信息
Ji Kun-Mei, Chen Jia-Jie, Gao Chen, Liu Xiao-Yu, Xia Li-Xin, Liu Zhi-Gang, Li Liang, Yang Suhan
机构信息
State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Nanhai Ave 3688, Shenzhen Guangdong 518060, PR China.
State Key Laboratory of Respiratory Disease for Allergy at Shengzhen University, School of Medicine, Shenzhen University, Nanhai Ave 3688, Shenzhen Guangdong 518060, PR China.
出版信息
Food Chem. 2011 Nov 15;129(2):541-545. doi: 10.1016/j.foodchem.2011.04.055. Epub 2011 Apr 30.
Food allergen labeling has not yet been implemented in China. Therefore, a gold immunochromatography assay (GICA) was developed using two monoclonal antibodies (mAb) against the peanut allergen Ara h1. The GICA was specific for standard peanut samples with a sensitivity of 10ng/ml. Peanut protein traces extracted from 124 food products imported and exported by China Customs were easily and rapidly detected by GICA. 68 food samples originally labeled as containing peanuts were positive for Ara h1 and 54 food samples labeled as not containing peanuts were negative for Ara h1, indicating that the labels from the manufacturers were accurate. However, 2 food samples labeled as not containing peanuts tested positive for Ara h1. The present GICA provides a fast, simple, semi-quantitative method for the determination of peanut allergens in foods. This detection system can be used to ensure the safety of food imported and exported by China Customs.
中国尚未实施食品过敏原标签制度。因此，利用两种抗花生过敏原Ara h1的单克隆抗体开发了一种金免疫层析测定法（GICA）。该GICA对标准花生样品具有特异性，灵敏度为10ng/ml。通过GICA可以轻松快速地检测从中国海关进出口的124种食品中提取的花生蛋白痕迹。68个最初标记含有花生的食品样本Ara h1呈阳性，54个标记不含花生的食品样本Ara h1呈阴性，这表明制造商的标签是准确的。然而，有2个标记不含花生的食品样本Ara h1检测呈阳性。目前的GICA为食品中花生过敏原的测定提供了一种快速、简单的半定量方法。该检测系统可用于确保中国海关进出口食品的安全。
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