Department of Science and Technology, Center on Biomolecular Electronics, Biomedical Instrumentation Section, National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi-110012, India.
Nanoscale. 2013 Apr 7;5(7):3043-51. doi: 10.1039/c3nr32242d.
Reduced graphene oxide (RGO) due to its excellent electrochemical properties and large surface area, has recently aroused much interest for electrochemical biosensing application. Here, the chemically active RGO has been synthesized and deposited onto an indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. This novel platform has been utilized for covalent attachment of the monoclonal antibodies of aflatoxin B1 (anti-AFB1) for food toxin (AFB1) detection. The electron microscopy, X-ray diffraction, and UV-visible studies reveal successful synthesis of reduced graphene oxide while the XPS and FTIR studies suggest its carboxylic functionalized nature. The electrochemical sensing results of the anti-AFB1/RGO/ITO based immunoelectrode obtained as a function of aflatoxin concentration show high sensitivity (68 μA ng(-1) mL cm(-2)) and improved detection limit (0.12 ng mL(-1)). The association constant (ka) for antigen-antibody interaction obtained as 5 × 10(-4) ng mL(-1) indicates high affinity of antibodies toward the antigen (AFB1).
由于具有优异的电化学性能和较大的比表面积，还原氧化石墨烯（RGO）最近在电化学生物传感应用中引起了广泛关注。在这里，通过电泳沉积技术将具有化学活性的 RGO 合成并沉积到涂有铟锡氧化物（ITO）的玻璃基底上。这个新平台已被用于共价连接黄曲霉毒素 B1 的单克隆抗体（抗 AFB1），以检测食品毒素（AFB1）。电子显微镜、X 射线衍射和紫外-可见研究表明成功合成了还原氧化石墨烯，而 XPS 和 FTIR 研究表明其具有羧酸官能化性质。作为黄曲霉毒素浓度函数获得的基于抗 AFB1/RGO/ITO 的免疫电极的电化学传感结果显示出高灵敏度（68 μA ng(-1) mL cm(-2)）和改善的检测限（0.12 ng mL(-1)）。作为抗原-抗体相互作用获得的结合常数（ka）为 5 × 10(-4) ng mL(-1)，表明抗体对抗原（AFB1）具有高亲和力。
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