Bagheri Mohammad, Amini Abdollah, Abdollahifar Mohammad-Amin, Ghoreishi Seyed Kamran, Piryaei Abbas, Pouriran Ramin, Chien Sufan, Dadras Sara, Rezaei Fatemehalsadat, Bayat Mohammad
1 School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran .
2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran .
Photomed Laser Surg. 2018 Aug;36(8):415-423. doi: 10.1089/pho.2018.4453. Epub 2018 Jul 6.
A lack of effective treatments still exists for patients suffering from diabetes mellitus. Photobiomodulation is proved as a beneficial therapeutic modality for wounds.
The aim of this study is to examine the effect of degranulation of mast cells and total number of mast cells in the remodeling step of an ischemic model of wound healing under the influence of photobiomodulation and conditioned medium (CM) from human bone marrow-derived mesenchymal stem cells (hBM-MSCs-CM), or CM, administered alone and or in combination.
Initially, type 1 diabetes mellitus was induced in 72 male adult rats. Then, after a month, one incision was made on the back of each rat. Subsequently, the rats were divided into four groups. The first group was considered as the control (placebo) group, the second group received CM, the third group received photobiomodulation, and the fourth group received photobiomodulation+CM. On days 4, 7, and 15, samples were extracted from the wound for histological and tensiometric examinations. The total number of mast cells, including the three types of mast cells, was counted by the stereological methods. The tensiometric properties of the repairing tissue were examined.
The administration of photobiomodulation and CM, alone or in combination, significantly increased the tensiometric properties within the healing wounds. Histologically, photobiomodulation+CM, CM, and photobiomodulation groups showed a significant decrease in the three types of mast cells and in the total number of mast cells compared with the control group on day 15.
We conclude that photobiomodulation and CM alone and or in combination significantly accelerated the healing process in a rat with a diabetic and ischemic wound, and significantly decreased the total number of mast cells and degranulation of mast cells. We suggest that the increased number of type 2 mast cells in the control group adversely affected the tensiometric properties of wounds in this group.
糖尿病患者仍然缺乏有效的治疗方法。光生物调节被证明是一种对伤口有益的治疗方式。
本研究旨在探讨在光生物调节以及人骨髓间充质干细胞条件培养基（hBM-MSCs-CM）单独或联合作用下，肥大细胞脱颗粒和肥大细胞总数在缺血性伤口愈合模型重塑阶段的影响。
首先，在72只成年雄性大鼠中诱导1型糖尿病。然后，一个月后，在每只大鼠背部做一个切口。随后，将大鼠分为四组。第一组作为对照组（安慰剂组），第二组接受条件培养基，第三组接受光生物调节，第四组接受光生物调节+条件培养基。在第4、7和15天，从伤口提取样本进行组织学和张力测定检查。通过体视学方法计数包括三种类型肥大细胞在内的肥大细胞总数。检查修复组织的张力特性。
单独或联合给予光生物调节和条件培养基，显著提高了愈合伤口的张力特性。组织学上，在第15天，光生物调节+条件培养基组、条件培养基组和光生物调节组与对照组相比，三种类型肥大细胞和肥大细胞总数均显著减少。
我们得出结论，单独或联合使用光生物调节和条件培养基可显著加速糖尿病缺血性伤口大鼠的愈合过程，并显著减少肥大细胞总数和肥大细胞脱颗粒。我们认为对照组中2型肥大细胞数量的增加对该组伤口的张力特性产生了不利影响。
