Kouhkheil Reza, Fridoni Mohammadjavad, Abdollhifar Mohammad-Amin, Amini Abdollah, Bayat Sahar, Ghoreishi Seyed Kamran, Chien Sufan, Kazemi Mahsa, Bayat Mohammad
Department of Anatomical Sciences, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran.
Photobiomodul Photomed Laser Surg. 2019 Nov;37(11):706-714. doi: 10.1089/photob.2019.4691. Epub 2019 Oct 7.
Numerous people suffer from diabetes mellitus (DM) and resultant diabetic foot ulcers (DFU), which lack effective treatment. Photobiomodulation (PBM) has accelerated wound healing in diabetic animals and patients in some studies. However, there is scant information on the number and activation state of skin mast cells (MCs) in PBM-treated diabetic wounds. We intend to assess the influence of the number of MCs and degranulation in the remodeling step of an infected wound model on wound strength and its microbial flora in a type 1 DM (T1DM) rat model by administration of PBM, condition medium (CM) derived from human bone marrow mesenchymal stem cells (hBMMSCs), and the combination of PBM+CM. We prepared CM by culturing hBMMSCs. T1DM was induced in 72 rats and, after 1 month, we created one excisional wound in each rat. All wounds were infected with methicillin-resistant (MRSA). We divided the rats into four groups: ( = 18): (i) control; (ii) PBM; (iii) CM, and (iv) PBM+CM. On days 4, 7, and 15, we conducted microbiological, tensiometrical, and stereological analyses. The type of MCs (T1MCs, T2MCs, or T3MCs) and total number of MCs (TOMCs) were counted by light microscopy. On day 15, the PBM+CM, PBM, and CM groups had significantly increased wound strength compared with the control group. There was a significant decrease in colony-forming units (CFU) at all time points in the PBM+CM and PBM groups. The PBM+CM and PBM groups had more stable MCs (T1MCs), less significant degranulated MCs (T2MCs), less significant disintegrated MCs (T3MCs), and less significant TOMCs compared with the control group at all time points. PBM+CM and PBM treatments significantly increased the healing process in an ischemic and MRSA-infected wound model of T1DM rats. PBM+CM and PBM significantly decreased both TOMCs and their degranulation, and significantly decreased CFU.
许多人患有糖尿病（DM）及由此引发的糖尿病足溃疡（DFU），而这些疾病缺乏有效的治疗方法。在一些研究中，光生物调节（PBM）已加速了糖尿病动物和患者伤口的愈合。然而，关于经PBM治疗的糖尿病伤口中皮肤肥大细胞（MCs）的数量和激活状态的信息却很少。我们打算通过给予PBM、源自人骨髓间充质干细胞（hBMMSCs）的条件培养基（CM）以及PBM + CM的组合，来评估在1型糖尿病（T1DM）大鼠模型中，感染性伤口模型重塑阶段MCs数量和脱颗粒对伤口强度及其微生物菌群的影响。我们通过培养hBMMSCs制备了CM。在72只大鼠中诱导出T1DM，1个月后，在每只大鼠身上制造一个切除伤口。所有伤口均感染耐甲氧西林金黄色葡萄球菌（MRSA）。我们将大鼠分为四组（每组 = 18只）：（i）对照组；（ii）PBM组；（iii）CM组；（iv）PBM + CM组。在第4、7和15天，我们进行了微生物学、张力测量和体视学分析。通过光学显微镜对MCs的类型（T1MCs、T2MCs或T3MCs）和MCs总数（TOMCs）进行计数。在第15天，与对照组相比，PBM + CM组、PBM组和CM组的伤口强度显著增加。在所有时间点，PBM + CM组和PBM组的菌落形成单位（CFU）均显著减少。与对照组相比，在所有时间点，PBM + CM组和PBM组具有更稳定的MCs（T1MCs）、脱颗粒不那么明显的MCs（T2MCs）、解体不那么明显的MCs（T3MCs）以及数量不那么多的TOMCs。PBM + CM和PBM治疗显著加快了T1DM大鼠缺血性和MRSA感染伤口模型的愈合过程。PBM + CM和PBM显著减少了TOMCs及其脱颗粒，并显著降低了CFU。
