Lee K, Tanaka M, Shigeno C, Yamamoto I, Ohta S, Rikimaru K, Hatanaka M, Konishi J
Department of Nuclear Medicine, Kyoto University School of Medicine, Japan.
Biochem Biophys Res Commun. 1990 May 16;168(3):905-11. doi: 10.1016/0006-291x(90)91114-8.
We examined the effects of epidermal growth factor (EGF) on the anchorage-dependent and -independent growth of four human squamous carcinoma cell lines that overexpress EGF receptors. While EGF inhibited anchorage-dependent growth, it stimulated anchorage-independent growth of all four cell lines tested. The results suggest that the proliferative responses to EGF are characterized by a preference for anchorage-independent, rather than -dependent growth, in cells overexpressing EGF receptors. Moreover, as EGF has been shown to stimulate the in vivo growth of squamous carcinoma cells overexpressing EGF receptors, it is also suggested that the in vitro EGF responsiveness of these cells in soft agar, but not in monolayer, better correlates with the in vivo EGF responsiveness.
我们研究了表皮生长因子（EGF）对四种过表达EGF受体的人鳞状癌细胞系的贴壁依赖性和非依赖性生长的影响。虽然EGF抑制贴壁依赖性生长，但它刺激了所有四种受试细胞系的贴壁非依赖性生长。结果表明，在过表达EGF受体的细胞中，对EGF的增殖反应的特征是优先选择贴壁非依赖性生长，而非贴壁依赖性生长。此外，由于已证明EGF可刺激过表达EGF受体的鳞状癌细胞的体内生长，还表明这些细胞在软琼脂中而非单层培养中的体外EGF反应性与体内EGF反应性更好相关。
