Lee K, Tanaka M, Hatanaka M, Kuze F
First Department of Medicine, Kyoto University, Japan.
Exp Cell Res. 1987 Nov;173(1):156-62. doi: 10.1016/0014-4827(87)90341-7.
The effects of epidermal growth factor (EGF) and transforming growth factor beta (TGF beta) on the growth of A431 epidermoid carcinoma cells were studied. Whereas the monolayer growth of A431 cells was inhibited by EGF, it was stimulated by TGF beta. Contrary to the effects on the monolayer growth, EGF stimulated the soft agar growth of A431 cells. The stimulatory effects of TGF beta on the anchorage-dependent growth were antagonized by EGF and those of EGF on anchorage-independent growth were antagonized by TGF beta. These results suggest that both factors not only convey the proliferative signals to A431 cells but also induce phenotypic changes, resulting in a preference for either anchorage-dependent or anchorage-independent growth. Moreover, as the stimulatory effects of EGF on the soft agar growth of A431 cells paralleled its reported stimulatory effects on their in vivo growth, it is also suggested that in vivo responses of cells to certain growth factors may correlate better with their responses in soft agar culture than with those in monolayer culture.
研究了表皮生长因子（EGF）和转化生长因子β（TGFβ）对A431表皮癌细胞生长的影响。虽然EGF抑制A431细胞的单层生长，但TGFβ却能刺激其生长。与对单层生长的影响相反，EGF刺激A431细胞在软琼脂中的生长。TGFβ对锚定依赖性生长的刺激作用被EGF拮抗，而EGF对非锚定依赖性生长的刺激作用被TGFβ拮抗。这些结果表明，这两种因子不仅向A431细胞传递增殖信号，还诱导表型变化，导致对锚定依赖性或非锚定依赖性生长的偏好。此外，由于EGF对A431细胞在软琼脂中生长的刺激作用与其对体内生长的刺激作用相似，还表明细胞对某些生长因子的体内反应与其在软琼脂培养中的反应可能比在单层培养中的反应相关性更好。
