Okuda T, Onda M, Tokunaga A, Sugisaki Y
First Department of Surgery, Nippon Medical School, Tokyo, Japan.
Surg Today. 1994;24(8):725-33. doi: 10.1007/BF01636779.
We recently established epidermal growth factor (EGF) receptor-hyperproducing human gastric cancer xenografts in nude mice. The present study was designed to examine whether the growth of a xenograft having 1,098 +/- 276 fmol/mg protein of EGF receptor would either be stimulated by the administration of EGF or inhibited by the removal of the submandibular glands (sialoadenectomy) which contain a large amount of EGF. A miniosmotic pump containing 2 micrograms or 20 micrograms of EGF was implanted on the back of the animals in the EGF stimulation experiments. The tumor growth was stimulated by the administration of EGF (P < 0.01), and the doubling time of the tumor was reduced relative to the controls (P < 0.01). Both the mitotic indices and the bromodeoxyuridine (BrdU)-labeling indices of the tumor were higher than those of the controls (P < 0.01). Tumor growth inhibited by the sialoadenectomy (P < 0.05) while the tumor doubling time was prolonged compared with the sham-operated mice (P < 0.05). These results suggest that the growth of a human gastric cancer xenograft may be modulated by EGF.
我们最近在裸鼠中建立了表皮生长因子（EGF）受体高表达的人胃癌异种移植模型。本研究旨在检测EGF受体含量为1,098±276 fmol/mg蛋白质的异种移植瘤的生长是否会受到EGF给药的刺激，或者是否会因切除含有大量EGF的下颌下腺（涎腺切除术）而受到抑制。在EGF刺激实验中，将含有2微克或20微克EGF的微型渗透泵植入动物背部。EGF给药刺激了肿瘤生长（P<0.01），且相对于对照组，肿瘤的倍增时间缩短（P<0.01）。肿瘤的有丝分裂指数和溴脱氧尿苷（BrdU）标记指数均高于对照组（P<0.01）。涎腺切除术后肿瘤生长受到抑制（P<0.05），而与假手术小鼠相比，肿瘤倍增时间延长（P<0.05）。这些结果表明，人胃癌异种移植瘤的生长可能受EGF调节。
