人类妊娠时胎盘后血淋巴细胞白细胞介素-2受体表达的降低

Reduction of interleukin-2-receptor expression on retroplacental blood lymphocytes in human pregnancy.

作者信息

Yoshida M, Kanzaki H, Kariya M, Emi N, Imai K, Okamoto N, Uchida A, Kasakura S, Mori T

机构信息

Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.

出版信息

Am J Reprod Immunol. 1990 Jan-Feb;22(1-2):1-3. doi: 10.1111/j.1600-0897.1990.tb01023.x.

DOI:10.1111/j.1600-0897.1990.tb01023.x

PMID:2346591

Abstract

Retroplacental blood lymphocytes (RPL) in human pregnancy were studied for proliferative response induced by recombinant interleukin-2 (rIL-2) and anti-Tac positive cells before and after PHA stimulation for 24 hr and compared with peripheral blood lymphocytes (PBL) of the same donor. Proliferation of RPL induced by IL-2 was significantly lower than that of PBL in all of six cases. In addition, flow cytometry analysis of IL-2 receptors (IL-2R) revealed that IL-2R expression of RPL after stimulation with PHA for 24 hr was significantly less than that of PBL in three of four cases and that RPL showed significantly fewer IL-2R than PBL even at resting state in one case. These results suggest that RPL seem likely to be composed of reduced numbers of lymphocytes bearing IL-2R of non-Tac protein and to have impaired capacity for the acquisition of high-affinity receptors for IL-2.

摘要

对人类妊娠时的胎盘后血淋巴细胞（RPL）进行了研究，观察其在PHA刺激24小时前后，由重组白细胞介素-2（rIL-2）和抗Tac阳性细胞诱导的增殖反应，并与同一供体的外周血淋巴细胞（PBL）进行比较。在所有6例中，IL-2诱导的RPL增殖明显低于PBL。此外，对IL-2受体（IL-2R）的流式细胞术分析显示，在4例中的3例中，PHA刺激24小时后RPL的IL-2R表达明显少于PBL，并且在1例中，即使在静止状态下，RPL的IL-2R也明显少于PBL。这些结果表明，RPL似乎由携带非Tac蛋白IL-2R的淋巴细胞数量减少所组成，并且获取IL-2高亲和力受体的能力受损。