人类妊娠时胎盘后血液淋巴细胞对丝裂原的反应性降低

Hyporeactivity to mitogens of retroplacental blood lymphocytes in human pregnancy.

作者信息

Yoshida M, Kanzaki H, Tokushige M, Sato S, Wang H S, Uchida A, Kasakura S, Mori T

机构信息

Department of Gynecology and Obstetrics, Kyoto University Faculty of Medicine, Japan.

出版信息

Immunol Lett. 1988 Mar;17(3):279-83. doi: 10.1016/0165-2478(88)90042-9.

DOI:10.1016/0165-2478(88)90042-9

PMID:3259540

Abstract

Retroplacental blood lymphocytes (RPL) obtained from women without complications at the term delivery were studied for proliferative response to mitogens and surface phenotype and were compared with autologous peripheral blood lymphocytes (PBL). Proliferation of RPL induced by phytohemagglutinin (PHA) was significantly lower than that of PBL in 11 of 13 cases. Also RPL proliferated poorly in response to concanavalin A (Con A) in 8 of 10 cases. On the other hand, flow cytometry analysis of T cell subsets revealed that RPL contain comparable or higher number of CD3+ cells and CD4+ cells and similar numbers of CD8+ cells as compared with PBL in most cases. These results indicate that T cell proliferative response in retroplacental space is impaired, which could be due to a functional defect of RPL.

摘要

对足月分娩无并发症女性所获取的胎盘后血淋巴细胞（RPL）进行了研究，检测其对有丝分裂原的增殖反应和表面表型，并与自体外周血淋巴细胞（PBL）进行比较。在13例中的11例中，植物血凝素（PHA）诱导的RPL增殖明显低于PBL。在10例中的8例中，RPL对刀豆球蛋白A（Con A）的增殖反应也较差。另一方面，T细胞亚群的流式细胞术分析显示，在大多数情况下，与PBL相比，RPL含有相当数量或更多的CD3 +细胞和CD4 +细胞，以及相似数量的CD8 +细胞。这些结果表明，胎盘后间隙中的T细胞增殖反应受损，这可能是由于RPL的功能缺陷所致。