Department of Virology II, National Institute of Infectious Diseases, Gakuen, Musashi-murayama, Tokyo, Japan.
J Virol. 2013 May;87(10):6031-6. doi: 10.1128/JVI.00444-13. Epub 2013 Mar 6.
Although the C-terminal 52 amino acids (C52aa) of hepatitis E virus (HEV) capsid are not essential for morphology, the C52aa-encoding region is required for replication. Transfection of a C52aa knockdown mutant showed transient growth, and the earliest population included a majority of noninfectious (possibly empty) particles and a minority of infectious particles with C-terminal capsid degradation. Finally, the complete revertant was generated reproducibly. C52aa is essential for the viral life cycle, promoting accurate encapsidation and stabilizing encapsidated particles.
尽管肝炎 E 病毒（HEV）衣壳的 C 末端 52 个氨基酸（C52aa）对形态不是必需的，但 C52aa 编码区是复制所必需的。转染 C52aa 敲低突变体显示出短暂的生长，最早的种群包括大多数无传染性（可能为空）颗粒和少数具有 C 末端衣壳降解的传染性颗粒。最后，可重复性地生成了完全回复突变体。C52aa 对病毒生命周期至关重要，促进了准确的包裹和稳定的包裹颗粒。
