Instituto de Biotecnología, CICVyA, CNIA, INTA-Castelar, Dr. N. Repetto y Los Reseros s/n, 1686, Hurlingham, Buenos Aires, Argentina.
Curr Microbiol. 2013 Aug;67(2):138-47. doi: 10.1007/s00284-013-0345-2. Epub 2013 Mar 8.
Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.
对来自阿根廷查科地区森林土壤样本的三个细菌群落的纤维素酶活性进行了表征。群落的系统发育分析揭示了包括不动杆菌属和假单胞菌属在内的两个主要高度支持的群组。所有三个群落都表现出纤维素酶活性。羧甲基纤维素酶（CMCase）和总纤维素酶活性进行了定量和定性研究，并对三个群落之间的最佳酶促条件进行了表征和比较。还分析了热稳定性和 pH 稳定性。基于其纤维素酶活性，选择了一个群落进行进一步的同工酶分析。我们检测到具有 CMCase 活性的 55 kDa 的特定蛋白质。在这项研究中，我们表明这些群落编码纤维素酶。这些酶可用于将木质纤维素生物质降解为简单成分，并用于不同的工业应用。
