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利用基因编码的 FRET 纳米传感器探索活体细胞中钼酸盐的动态变化。

Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor.

机构信息

Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan.

出版信息

PLoS One. 2013;8(3):e58175. doi: 10.1371/journal.pone.0058175. Epub 2013 Mar 5.

DOI:10.1371/journal.pone.0058175
PMID:23472155
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3589368/
Abstract

Molybdenum (Mo) is an essential trace element for almost all living organisms including animals. Mo is used as a catalytic center of molybdo-enzymes for oxidation/reduction reactions of carbon, nitrogen, and sulfur metabolism. Whilst living cells are known to import inorganic molybdate oxyanion from the surrounding environment, the in vivo dynamics of cytosolic molybdate remain poorly understood as no appropriate indicator is available for this trace anion. We here describe a genetically encoded Förester-resonance-energy-transfer (FRET)-based nanosensor composed of CFP, YFP and the bacterial molybdate-sensor protein ModE. The nanosensor MolyProbe containing an optimized peptide-linker responded to nanomolar-range molybdate selectively, and increased YFP:CFP fluorescence intensity ratio by up to 109%. By introduction of the nanosensor, we have been able to successfully demonstrate the real-time dynamics of molybdate in living animal cells. Furthermore, time course analyses of the dynamics suggest that novel oxalate-sensitive- and sulfate-resistant- transporter(s) uptake molybdate in a model culture cell.

摘要

钼（Mo）是几乎所有生物体（包括动物）的必需微量元素。Mo 用作钼酶的催化中心，用于碳、氮和硫代谢的氧化/还原反应。虽然已知活细胞从周围环境中导入无机钼酸盐阴离子，但由于没有合适的指示剂用于这种痕量阴离子，因此细胞浆中钼酸盐的体内动力学仍知之甚少。我们在这里描述了一种由 CFP、YFP 和细菌钼酸盐传感器蛋白 ModE 组成的遗传编码的 Förester 共振能量转移（FRET）纳米传感器。含有优化肽接头的纳米传感器 MolyProbe 对纳米摩尔范围内的钼酸盐选择性响应，并使 YFP：CFP 荧光强度比增加高达 109%。通过引入纳米传感器，我们已经能够成功地证明活动物细胞中钼酸盐的实时动态。此外，对动力学的时程分析表明，新型草酸盐敏感和硫酸盐抗性转运体在模型培养细胞中摄取钼酸盐。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/cbe58257488b/pone.0058175.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/452a3627ebe9/pone.0058175.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/1e52ffbbf9f3/pone.0058175.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/d3063c294432/pone.0058175.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/9eeedaf1b809/pone.0058175.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/cbe58257488b/pone.0058175.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/452a3627ebe9/pone.0058175.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/1e52ffbbf9f3/pone.0058175.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/d3063c294432/pone.0058175.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/9eeedaf1b809/pone.0058175.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ac1/3589368/cbe58257488b/pone.0058175.g005.jpg

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本文引用的文献

1
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2
Molybdenum enzymes in higher organisms.高等生物中的钼酶。
Coord Chem Rev. 2011 May 1;255(9-10):1179-1205. doi: 10.1016/j.ccr.2010.11.034.
3
Algae and humans share a molybdate transporter.藻类和人类共享一种钼酸盐转运蛋白。
钼等微量营养素在妊娠并发症和胎盘健康方面是否发挥作用？
Nutrients. 2023 Jul 27;15(15):3348. doi: 10.3390/nu15153348.
4
Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives.利用基因编码传感器监测植物中的营养物质：成就与展望。
Plant Physiol. 2023 Aug 31;193(1):195-216. doi: 10.1093/plphys/kiad337.
5
Genetically encoded fluorescent sensors for metals in biology.用于生物学中金属的基因编码荧光传感器。
Curr Opin Chem Biol. 2023 Jun;74:102284. doi: 10.1016/j.cbpa.2023.102284. Epub 2023 Mar 12.
6
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Molecules. 2022 Jun 8;27(12):3691. doi: 10.3390/molecules27123691.
7
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ACS Omega. 2020 Nov 12;5(46):30306-30314. doi: 10.1021/acsomega.0c04868. eCollection 2020 Nov 24.
8
A Non-Invasive Tool for Real-Time Measurement of Sulfate in Living Cells.一种用于实时测量活细胞中硫酸盐的非侵入性工具。
Int J Mol Sci. 2020 Apr 7;21(7):2572. doi: 10.3390/ijms21072572.
9
Development of heme protein based oxygen sensing indicators.血红素蛋白基氧传感指示剂的研制。
Sci Rep. 2018 Aug 7;8(1):11849. doi: 10.1038/s41598-018-30329-5.
10
Application of FRET probes in the analysis of neuronal plasticity.荧光共振能量转移探针在神经元可塑性分析中的应用。
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Proc Natl Acad Sci U S A. 2011 Apr 19;108(16):6420-5. doi: 10.1073/pnas.1100700108. Epub 2011 Apr 4.
4
Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.用于追踪活细胞中信号转导动态的基因编码荧光生物传感器。
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5
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6
Visualization of ATP levels inside single living cells with fluorescence resonance energy transfer-based genetically encoded indicators.利用基于荧光共振能量转移的基因编码指示剂对单个活细胞内的ATP水平进行可视化。
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15651-6. doi: 10.1073/pnas.0904764106. Epub 2009 Aug 31.
7
Molybdenum cofactors, enzymes and pathways.钼辅因子、酶与代谢途径。
Nature. 2009 Aug 13;460(7257):839-47. doi: 10.1038/nature08302.
8
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9
A high-affinity molybdate transporter in eukaryotes.真核生物中的一种高亲和力钼酸盐转运蛋白。
Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):20126-30. doi: 10.1073/pnas.0704646104. Epub 2007 Dec 5.
10
An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.一种拟南芥高亲和力钼酸盐转运蛋白，是从土壤中高效吸收钼酸盐所必需的。
Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18807-12. doi: 10.1073/pnas.0706373104. Epub 2007 Nov 14.