Mahmood Khalid, Mohammad Noor
Department of Medicine, Postgraduate Medical Institute, Lady Reading Hospital, Peshawar, Pakistan.
J Ayub Med Coll Abbottabad. 2011 Oct-Dec;23(4):18-21.
To determine the frequency of various genotypes of Hepatitis C virus in District Buner, Swat.
This Descriptive case series study was conducted at District Headquarter Hospital Daggar, and Bilal Medical Trust, Pir Baba, Swat, Pakistan from January 2007 to June 2008. A total of 400 patients, 154 Male and 246 Female aged 16-65 years (Mean age 31 +/- 13 years) without clinical and ultrasonographic evidence of cirrhosis, and with positive Hepatitis C Virus (HCV) antibodies by ELIZA, and HCV RNA detected by PCR were included. Hepatitis C virus genotypes were checked in serum by real time PCR (RT-PCR).
Genotype 3 was the most common type accounting for 61.5% of the study population. Amongst the subtypes, 182 (45.5%) had genotype 3a and 64 (16%) had genotype 3b. Thirty-five (8.7%) patients had genotype 1a, 12 (3%) had genotype 1b. Twenty-six (6.5%) patients had genotype 2a. Four (1%) patients had genotype 2b. Three (0.75%) patients had genotype 4a, and 1 (0.25%) had genotype 6a. Sixty-four (16%) patients had mixed infections. Forty (10%) patients had genotype 3a and 3b, 12 (3%) patients had genotype 1a, 1b, and 9 (2.25%) patients had genotype 2a and 1c, 2 (0.5%) had genotypela, 3a and 1 patient (0.25%) had genotype 1a, 3b. Nine (2.25%) patients had untypable genotype.
Genotype 3 with its subtypes 3a and 3b is the commonest Hepatitis C virus present in District Buner, Swat. The others in order of decreasing frequency are genotype 1a, 2a and mixed types.
确定斯瓦特布内尔地区丙型肝炎病毒各种基因型的出现频率。
本描述性病例系列研究于2007年1月至2008年6月在巴基斯坦斯瓦特的达格加地区总部医院以及皮尔雅巴巴的比拉尔医疗信托医院开展。共纳入400例患者，年龄在16至65岁之间（平均年龄31±13岁），其中男性154例，女性246例，无肝硬化的临床及超声证据，酶联免疫吸附测定法检测丙型肝炎病毒（HCV）抗体呈阳性，且聚合酶链反应检测到HCV RNA。通过实时聚合酶链反应（RT-PCR）检测血清中的丙型肝炎病毒基因型。
基因型3是最常见的类型，占研究人群的61.5%。在各亚型中，182例（45.5%）为基因型3a，64例（16%）为基因型3b。35例（8.7%）患者为基因型1a，12例（3%）为基因型1b。26例（6.5%）患者为基因型2a。4例（1%）患者为基因型2b。3例（0.75%）患者为基因型4a，1例（0.25%）为基因型6a。64例（16%）患者存在混合感染。40例（10%）患者为基因型3a和3b，12例（3%）患者为基因型1a、1b，9例（2.25%）患者为基因型2a和1c，2例（0.5%）为基因型1a、3a，1例患者（0.25%）为基因型1a、3b。9例（2.25%）患者的基因型无法分型。
基因型3及其亚型3a和3b是斯瓦特布内尔地区最常见的丙型肝炎病毒。按出现频率递减顺序排列，其他类型依次为基因型1a、2a和混合类型。
