检测在巴基斯坦旁遮普省不同地区流行的丙型肝炎病毒基因型。
Determination of hepatitis C virus genotypes circulating in different districts of Punjab (Pakistan).
机构信息
Departments of aMicrobiology bGenetics, Hazara University (Garden Campus), Mansehra cDepartment of Medicine, Khyber Medical University, Peshawar dDepartment of Biotechnology, University of Malakand, Khyber Pakhtunkhwa eDepartment of Medicine, Mardan Complex Hospital, Mardan fNational Centre of Excellence in Molecular Biology, Division of Molecular Virology and Molecular Diagnostics, University of the Punjab gGenome Center for Molecular Diagnosis & Research, Lahore, Pakistan.
出版信息
Eur J Gastroenterol Hepatol. 2014 Jan;26(1):59-64. doi: 10.1097/MEG.0b013e328362dc3f.
INTRODUCTION
Hepatitis C virus (HCV) is a principal cause of severe liver diseases worldwide and a possible source of significant morbidity and fatality in the long term. Information on the genotype is more significant because it has prognostic value in the response to antiviral therapy.
OBJECTIVES
This study aimed to determine the frequency of various HCV genotypes circulating in the different districts of Punjab and to show the pattern distribution of HCV genotypes in different age groups and sexes.
MATERIALS AND METHODS
A total of 542 HCV-positive patients were selected from various districts of the province and were subjected to an HCV genotype-specific assay. Among 542 positive patients, 300 (55.35%) were men and 242 (44.65%) were women. There were 245 (45.20%), 61 (11.25%), 56 (10.33%), 56 (10.33%), 53 (9.77%), 27 (5%), 20 (3.69%), 16 (3%), and eight (1.47%) HCV-positive patients from Lahore, Sargodha, Multan, Toba Tek Singh, Faisalabad, Rawalpindi, Mandi Bahauddin Gujranwala, and Sahiwal districts, respectively.
RESULTS
Of a total of 542 serum samples analyzed, 476 (88%) were successfully genotyped whereas 66 (12%) samples were undetermined genotypes. Among the typable genotypes, 1a was found in 37 (7%), 1b in 18 (3%), 3a in 386 (71%), and genotype 3b in five (1%) patients. Thirty patients (6%) were identified to be infected with mixed HCV genotypes. Genotypes 3a (P=0.0001), 1a (P=0.001), and untypable genotypes (P=0.03) were circulating significantly in all the studied districts. All the genotypes were distributed evenly in male and female patients. The most affected age range of patients was 21-40 years as compared with teenage and older age groups.
CONCLUSION
The study found a significantly high prevalence of HCV among the patients of Punjab. In addition, genotype 1a was found to be a significantly and rapidly increasing genotype in the study area. It appears that HCV-3a (the most prevalent genotype) may be replaced by genotype 1a. If this occurs, it will make the present scenario more complex in terms of response to therapy and economic burden on the health sector of Pakistan. HCV infection is more common in the age group of 21-40 years. All the genotypes were distributed at the same frequency in men and women.
简介
丙型肝炎病毒（HCV）是全球严重肝脏疾病的主要病因之一，也是长期内导致重大发病率和死亡率的潜在因素。有关基因型的信息更为重要，因为它对抗病毒治疗的反应具有预后价值。
目的
本研究旨在确定旁遮普省不同地区循环的各种 HCV 基因型的频率，并展示不同年龄组和性别人群中 HCV 基因型的分布模式。
材料和方法
从该省的各个地区选择了 542 名 HCV 阳性患者，并对其进行了 HCV 基因型特异性检测。在 542 名阳性患者中，男性 300 名（55.35%），女性 242 名（44.65%）。来自拉合尔、萨戈达、木尔坦、托巴 Tek 辛格、费萨拉巴德、拉瓦尔品第、曼迪巴哈丁古尔赞瓦拉和锡亚瓦尔地区的 HCV 阳性患者分别为 245 名（45.20%）、61 名（11.25%）、56 名（10.33%）、56 名（10.33%）、53 名（9.77%）、27 名（5%）、20 名（3.69%）、16 名（3%）和 8 名（1.47%）。
结果
在分析的总共 542 份血清样本中，成功确定基因型的有 476 份（88%），66 份（12%）样本为不确定基因型。在可分型的基因型中，发现 1a 型 37 例（7%）、1b 型 18 例（3%）、3a 型 386 例（71%）和 3b 型 5 例（1%）。30 名患者（6%）被鉴定为混合 HCV 基因型感染。基因型 3a（P=0.0001）、1a（P=0.001）和无法分型的基因型（P=0.03）在所有研究地区均有显著循环。所有基因型在男性和女性患者中分布均匀。受影响最严重的年龄范围是 21-40 岁，其次是青少年和老年人群体。
结论
本研究发现旁遮普省的 HCV 患者患病率显著较高。此外，研究区域内发现 1a 基因型呈显著快速增长趋势。似乎 HCV-3a（最常见的基因型）可能被 1a 基因型取代。如果发生这种情况，这将使巴基斯坦卫生部门的治疗反应和经济负担方面的现状更加复杂。HCV 感染在 21-40 岁年龄组更为常见。所有基因型在男性和女性中的分布频率相同。
Zotero 插件