Benfey P N, Ren L, Chua N H
Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021.
EMBO J. 1990 Jun;9(6):1685-96. doi: 10.1002/j.1460-2075.1990.tb08292.x.
We have analyzed expression conferred by five subdomains of the cauliflower mosaic virus (CaMV) 35S enhancer in mature transgenic plants. Expression was detected from subdomains that gave no expression at earlier stages of development indicating developmental regulation of expression and confirming the modular organization of the enhancer. In several cases the expression patterns are highly restricted in cell type, providing useful markers for developmental studies. Comparison of expression patterns conferred by various combinations of 35S enhancer cis-elements suggests that synergistic interactions among cis-elements may play an important role in defining tissue-specific expression. This has implications for the nature of a cis-element combinatorial code that could define expression throughout development.
我们分析了花椰菜花叶病毒（CaMV）35S增强子的五个亚结构域在成熟转基因植物中赋予的表达。在发育早期无表达的亚结构域检测到了表达，这表明表达具有发育调控性，并证实了增强子的模块化组织。在几种情况下，表达模式在细胞类型上受到高度限制，为发育研究提供了有用的标记。对由35S增强子顺式元件的各种组合赋予的表达模式进行比较表明，顺式元件之间的协同相互作用可能在定义组织特异性表达中起重要作用。这对可能在整个发育过程中定义表达的顺式元件组合密码的性质具有启示意义。
