Benfey P N, Ren L, Chua N H
Laboratory of Plant Molecular Biology, Rockefeller University, New York, NY 10021.
EMBO J. 1990 Jun;9(6):1677-84. doi: 10.1002/j.1460-2075.1990.tb08291.x.
The cauliflower mosaic virus (CaMV) 35S enhancer is able to confer strong constitutive expression in plants. We have previously defined two domains within this enhancer that can confer different tissue-specific expression patterns throughout development. We show here that the upstream domain (B) has a modular organization. It contains at least five subdomains that are able to confer distinct expression patterns when fused to the downstream domain (A). When fused to a minimal promoter only three of the five subdomains give any expression in the early stages of plant development. Comparison of the expression patterns conferred by the subdomains alone, in combination with the downstream domain or in combination with other subdomains provides evidence for synergistic interactions among cis-elements within the 35S enhancer.
花椰菜花叶病毒（CaMV）35S增强子能够在植物中赋予强组成型表达。我们之前已经在该增强子中定义了两个结构域，它们在整个发育过程中能够赋予不同的组织特异性表达模式。我们在此表明，上游结构域（B）具有模块化组织。它包含至少五个亚结构域，当与下游结构域（A）融合时能够赋予不同的表达模式。当仅与最小启动子融合时，五个亚结构域中的三个在植物发育早期产生任何表达。单独的亚结构域、与下游结构域组合或与其他亚结构域组合所赋予的表达模式的比较，为35S增强子内顺式元件之间的协同相互作用提供了证据。
