脂质膜的纳米力学性质受乙醇的存在显著影响。
The nanomechanical properties of lipid membranes are significantly influenced by the presence of ethanol.
机构信息
IMETUM, Physik-Department, E22a, Technische Universität München, Munich, Germany.
出版信息
Biophys J. 2013 Mar 5;104(5):1049-55. doi: 10.1016/j.bpj.2013.01.021.
Abstract
Ethanol has a profound impact on biological systems and is moreover used in various medical and nonmedical applications. Its interaction with the lipid part of biological membranes has been the subject of intensive studies, but surprisingly, to our knowledge, no study has examined the influence of ethanol on lipid bilayer nanomechanics. We performed atomic force microscopy-based measurements to assess the influence of ethanol on the nanomechanical properties of fluid supported lipid bilayers. Ethanol significantly reduces membrane stability, bilayer thickness, Young's modulus, area stretch modulus, and bending stiffness. Altogether, our data suggest that ethanol addition to supported lipid bilayers supports both the hydrophobic and the hydrophilic permeation pathways by a decrease of bilayer thickness and reduced stability, respectively.
摘要
乙醇对生物系统有深远的影响，并且在各种医学和非医学应用中都有使用。它与生物膜的脂质部分的相互作用一直是密集研究的主题，但令人惊讶的是，据我们所知，没有研究检查过乙醇对脂质双层纳米力学的影响。我们进行了基于原子力显微镜的测量，以评估乙醇对流体支撑脂质双层的纳米力学性质的影响。乙醇显著降低了膜的稳定性、双层厚度、杨氏模量、面积拉伸模量和弯曲刚度。总的来说，我们的数据表明，乙醇的添加支持了疏水性和亲水性渗透途径，这分别是通过降低双层厚度和降低稳定性来实现的。
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