Okoko Tebekeme
Department of Biochemistry, Faculty of Basic Medical Sciences, Niger Delta University, PMB 71 Wilberforce Island, Bayelsa State, Nigeria.
J Genet Eng Biotechnol. 2018 Dec;16(2):485-490. doi: 10.1016/j.jgeb.2018.02.004. Epub 2018 Feb 17.
The abilities of kolaviron and selenium (either separately or in combination) to prevent hydrogen peroxide-induced alterations in cell viability and activation were investigated. The cell line U937 was incubated with the antioxidants (i.e. kolaviron or selenium) for 24 h before exposure to hydrogen peroxide and cell viability was assessed via trypan blue dye exclusion assay. The U937 cells were also transformed to the macrophage form, incubated with the antioxidants before exposure to hydrogen peroxide. Subsequently, production of nitric oxide and pro-inflammatory cytokines were assessed as indices of macrophage activation. The myoblast cell line H9c2 was also incubated with Se and kolaviron for 24 h before exposure to hydrogen peroxide. Cell viability and generation of reactive oxygen species (ROS) were assessed via MTT and DCHF assays. The results revealed that hydrogen peroxide significantly reduced ( < 0.05) the viability of U937 cells which was ameliorated by kolaviron and selenium. Kolaviron and selenium also reduced hydrogen peroxide-induced secretion of nitric oxide, TNF-α, IL-1 and IL-6 by transformed U937 cells. Hydrogen peroxide also significantly reduced ( < 0.05) the viability of H9c2 cells which was significantly restored by kolaviron. Though selenium had no effect on the proliferation of H9c2 cells, co-treatment with kolaviron significantly reduced hydrogen peroxide-induced alterations. Both kolaviron and selenium also reduced hydrogen peroxide-mediated ROS production by H9c2 cells. In all cases, the combined action of kolaviron and selenium offered greater amelioration of the hydrogen peroxide-induced alterations than their separate effects ( < 0.05) but may not be synergistic or additive.
研究了可乐维隆和硒（单独或联合使用）预防过氧化氢诱导的细胞活力改变和激活的能力。在暴露于过氧化氢之前，将U937细胞系与抗氧化剂（即可乐维隆或硒）孵育24小时，并通过台盼蓝染料排除试验评估细胞活力。U937细胞也被转化为巨噬细胞形式，在暴露于过氧化氢之前与抗氧化剂孵育。随后，评估一氧化氮和促炎细胞因子的产生作为巨噬细胞激活的指标。在暴露于过氧化氢之前，成肌细胞系H9c2也与硒和可乐维隆孵育24小时。通过MTT和DCHF试验评估细胞活力和活性氧（ROS）的产生。结果显示，过氧化氢显著降低（<0.05）U937细胞的活力，而可乐维隆和硒可改善这种情况。可乐维隆和硒还减少了过氧化氢诱导的转化U937细胞分泌一氧化氮、TNF-α、IL-1和IL-6。过氧化氢也显著降低（<0.05）H9c2细胞的活力，而可乐维隆可显著恢复这种活力。虽然硒对H9c2细胞的增殖没有影响，但与可乐维隆联合处理可显著减少过氧化氢诱导的改变。可乐维隆和硒还减少了过氧化氢介导的H9c2细胞ROS产生。在所有情况下，可乐维隆和硒的联合作用比它们单独的作用更能改善过氧化氢诱导的改变（<0.05），但可能不是协同或相加的。
