Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, Shanghai 200136, China.
Arch Microbiol. 2013 May;195(5):313-22. doi: 10.1007/s00203-013-0880-z. Epub 2013 Mar 10.
Two 16S rRNA gene clone libraries Cores 1U and 2U were constructed using two ice core samples collected from Austre Lovénbreen glacier in Svalbard. The two libraries yielded a total of 262 clones belonging to 59 phylotypes. Sequences fell into 10 major lineages of the domain Bacteria, including Proteobacteria (alpha, beta, gamma and delta subdivisions), Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria, Deinococcus-Thermus, Chloroflexi, Planctomycetes, Cyanobacteria and candidate division TM7. Among them, Bacteroidetes, Actinobacteria, Alphaproteobacteria and Cyanobacteria were most abundant. UniFrac data showed no significant differences in community composition between the two ice cores. A total of nineteen bacterial strains from the genera Pseudoalteromonas and Psychrobacter were isolated from the ice cores. Phylogenetic and phenotypic analyses revealed a close relationship between the ice core isolates and bacteria in marine environments, indicating a wide distribution of some bacterial phylotypes in both terrestrial and marine ecosystems.
利用从斯瓦尔巴群岛的奥斯特洛夫冰川采集的两个冰芯样本构建了两个 16S rRNA 基因克隆文库 Core 1U 和 2U。这两个文库共产生了 262 个克隆，属于 59 个种型。序列分为 10 个细菌域的主要谱系，包括变形菌门（α、β、γ 和 δ 亚门）、拟杆菌门、放线菌门、厚壁菌门、酸杆菌门、Deinococcus-Thermus 门、绿弯菌门、浮霉菌门、蓝细菌门和候选门 TM7。其中，拟杆菌门、放线菌门、α-变形菌门和蓝细菌门最为丰富。UniFrac 数据显示两个冰芯之间的群落组成没有显著差异。从冰芯中总共分离出了来自假交替单胞菌属和嗜冷杆菌属的 19 个细菌菌株。系统发育和表型分析表明，冰芯分离物与海洋环境中的细菌密切相关，表明一些细菌种型在陆地和海洋生态系统中广泛分布。
