Wei Zhong-Bao, Sun Jia-Ming, Li Peng-Fei, Wang Shuai, Zhang Hui, Lin Zhe
Jilin Modern Medical and Biopharmaceutical Base Office, Changchun 130041, China.
Zhongguo Zhong Yao Za Zhi. 2012 Dec;37(23):3591-4.
To study the antioxidant constituents from the root of Rubus crataegifolius.
The constituents isolation and purification from the root of R. crataegifolius was carried by reported column chromatography including silica gel, toyopearl, and their structures were elucidated on the basis of spectral compounds. DPPH method was used to evaluate the free radical scavenging activity of the isolated compounds.
Nine compounds were isolated from the root of R. crataegifolius, and their structures were identified as follow: euscaphic acid (1), kaempferol-3-O-beta-D-galactopyranoside (2), tormentic acid (3), 2alpha, 19alpha, 24-trihydroxyurs-12-ene-3-oxo-28-acid (4) , 2alpha-hydroxy-oleanolic acid (5), ursolic acid (6), daucosterol (7), beta-sitosterol (8) and polydatin (9). By experiment of antioxidant activity, the result showed compounds 2 and 9 revealed DPPH free radical scavenging rates were 95.60% and 75.23% at the concentration of 50 mg x L(-1).
Compounds 1-8 were isolated from this plant for the first time, and compounds 2 and 9 showed the significant antioxidant activity.
研究山楂叶悬钩子根的抗氧化成分。
采用硅胶柱色谱、Toyopearl柱色谱等报道的柱色谱法对山楂叶悬钩子根中的成分进行分离纯化，并根据光谱化合物对其结构进行鉴定。采用DPPH法评价分离得到的化合物的自由基清除活性。
从山楂叶悬钩子根中分离得到9个化合物，其结构鉴定如下：委陵菜酸（1）、山柰酚-3-O-β-D-吡喃半乳糖苷（2）、 tormentic酸（3）、2α,19α,24-三羟基乌苏-12-烯-3-氧代-28-酸（4）、2α-羟基齐墩果酸（5）、熊果酸（6）、胡萝卜苷（7）、β-谷甾醇（8）和白藜芦醇苷（9）。通过抗氧化活性实验，结果表明化合物2和9在50mg·L⁻¹浓度下对DPPH自由基的清除率分别为95.60%和75.23%。
化合物1-8为首次从该植物中分离得到，化合物2和9具有显著的抗氧化活性。
