Fu Zhenna, Wei Zhenzhen, Miao Mingsan
Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
College of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China.
Saudi J Biol Sci. 2018 Mar;25(3):487-492. doi: 10.1016/j.sjbs.2017.08.009. Epub 2017 Aug 18.
To study the effect of raspberry total flavonoids on perimenopausal model in mice. Blank group, sham operation, and the rest of the mice made the menopausal model. Choose 72 mice castrated completely random divided into 6 groups for the experiment, respectively: model group, gengnianan (GNA) capsule group, soybean isoflavone soft (SIS) capsule group, high, mid and low dose group of total flavonoids of raspberry (TFR). Animals in each group were given the corresponding drugs tenth days after operation, and were given intragastrical administration of once a day for continuous administration of 21 days. Each group of mice in the administration of 18 days to determine the number of autonomic activities within 5 min, in the administration of 19-20 days to determine the incubation of the mice first entry into the darkroom and the number of shocks into the darkroom within 5 min. At 2 h after the last administration (fasting for 12 h), mice were sacrificed and serum was collected. Serum levels of E2, T, LH and FSH were measured. Dissect the uterus, uterus, thymus and spleen. Weigh the wet weight and calculate the organ index, the morphological changes of uterus, thymus and spleen were observed. The results showed that the TFR had a good therapeutic effect on the perimenopausal model of mice after giving a high, mid and low dose of raspberry flavonoids for some time.
研究树莓总黄酮对小鼠围绝经期模型的影响。设空白组、假手术组，其余小鼠制备绝经模型。选取72只去势小鼠完全随机分为6组进行实验，分别为：模型组、更年安（GNA）胶囊组、大豆异黄酮软胶囊（SIS）组、树莓总黄酮高、中、低剂量组（TFR）。各组动物于术后第10天给予相应药物，每日灌胃1次，连续给药21天。每组小鼠在给药第18天时测定5分钟内自主活动次数，在给药第19 - 20天时测定小鼠首次进入暗室的潜伏期及5分钟内进入暗室的电击次数。末次给药2小时后（禁食12小时），处死小鼠并采集血清。测定血清中E2、T、LH和FSH水平。解剖子宫、子宫、胸腺和脾脏。称取湿重并计算脏器指数，观察子宫、胸腺和脾脏的形态变化。结果显示，给予高、中、低剂量树莓黄酮一段时间后，TFR对小鼠围绝经期模型有良好的治疗作用。
