State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Chem Commun (Camb). 2013 Apr 18;49(30):3137-9. doi: 10.1039/c3cc40906f. Epub 2013 Mar 12.
A highly sensitive and convenient chemiluminescence (CL) turn-on assay for protease without any label and synthesis has been developed. Cytochrome c was digested by a protease, and heme was released as a peptide-heme conjugate, which greatly enhanced the luminol-H2O2 CL reaction.
开发了一种无需任何标记和合成的高灵敏度、简便的化学发光（CL）法用于蛋白酶的检测。蛋白酶将细胞色素 c 进行酶切，血红素作为肽-血红素结合物释放出来，极大地增强了鲁米诺-H2O2 的 CL 反应。
