Dogru Serkan, Dogru Hatice Yilmaz, Butun Ilknur, Arici Akgul, Benli Ismail, Karaman Tugba, Tapar Hakan, Sahin Aynur, Karaman Serkan, Arici Semih, Ozsoy Asker Zeki
Department of Anesthesiology and Reanimation, Gaziosmanpasa University, Medical Faculty, Tokat, Turkey.
Department of Obstetrics and Gynecology, Gaziosmanpasa University, Medical Faculty, Tokat, Turkey.
J Pak Med Assoc. 2017 Jun;67(6):877-883.
To determine the effects of sevoflurane by inhalation on female reproductive hormones and ovarian tissues.
This experimental study was conducted at the Gaziosmanpasa University, Tokat, Turkey, and comprised Wistar-Albino female rats. The rats were divided into six groups; one control and five study groups. The control group (C) received 2 L/min O2 in 18 min/day for seven days; the first study group (S1) received 1 minimum alveolar concentration sevoflurane + 2 L/min O2 in 18 min/day for seven days; the second group (S2) received 1 minimum alveolar concentration sevoflurane + 2 L/min O2 in 18 min/day for seven days and no treatment for the following seven days; the third group (S3) received 1 minimum alveolar concentration sevoflurane + 2 L/min O2 in 18 min/day for 14 days; the fourth group (S4) received 1 minimum alveolar concentration sevoflurane + 2 L/min O2 in 18 min/day for 14 days and no treatment for the following seven days; and the fifth group (S5) received 1 minimum alveolar concentration sevoflurane + 2 L/min O2 in 18 min/day for 14 days and no treatment for the following 14 days. The duration of the study was 28 days in February 2015. Reproductive system hormone levels were analysed and histological assessment of the ovaries was performed. SPSS 20 was used for data analysis.
Of the 30 rats, there were 5(16.7%) in each group. Histological injury scores in S2, S3, S4, and S5 were significantly higher than in C (p=0.016, p=0.008, p=0.016 and p=0.032, respectively). The hormone levels belonging to follicle stimulating hormone, luteinising hormone, estradiol and progesterone revealed significant alterations in all groups (p<0.05).
Chronic exposure to sevoflurane negatively affected the histological structure of the ovary and hormonal regulation.
确定吸入七氟醚对雌性生殖激素和卵巢组织的影响。
本实验研究在土耳其托卡特的加济奥斯曼帕夏大学进行，实验对象为Wistar - 白化雌性大鼠。大鼠被分为六组；一组为对照组，五组为研究组。对照组（C）每天18分钟接受2升/分钟的氧气，持续7天；第一研究组（S1）每天18分钟接受1个最低肺泡有效浓度的七氟醚 + 2升/分钟的氧气，持续7天；第二组（S2）每天18分钟接受1个最低肺泡有效浓度的七氟醚 + 2升/分钟的氧气，持续7天，接下来7天不进行处理；第三组（S3）每天18分钟接受1个最低肺泡有效浓度的七氟醚 + 2升/分钟的氧气，持续14天；第四组（S4）每天18分钟接受1个最低肺泡有效浓度的七氟醚 + 2升/分钟的氧气，持续14天，接下来7天不进行处理；第五组（S5）每天18分钟接受1个最低肺泡有效浓度的七氟醚 + 2升/分钟的氧气，持续14天，接下来14天不进行处理。研究持续时间为2015年2月的28天。分析生殖系统激素水平并对卵巢进行组织学评估。使用SPSS 20进行数据分析。
30只大鼠中，每组有5只（16.7%）。S2、S3、S4和S5组的组织学损伤评分显著高于C组（分别为p = 0.016、p = 0.008、p = 0.016和p = 0.032）。所有组中促卵泡激素、促黄体生成素、雌二醇和孕酮的激素水平均有显著变化（p < 0.05）。
长期暴露于七氟醚对卵巢的组织结构和激素调节产生负面影响。
