Reproductive Medicine Center, for The First Affiliated Hospital of Sun Yat-sen University, Key Laboratory of Reproductive Medicine of Guangdong Province, Sun Yat-sen University, Guangzhou, China.
Am J Reprod Immunol. 2013 Sep;70(3):221-9. doi: 10.1111/aji.12111. Epub 2013 Mar 11.
To determine the presence of antinuclear antibodies (ANAs) in follicular fluid (FF) and embryos as to their potential impact on IVF outcome.
A total of 100 women presenting to IVF/ICSI program were recruited in this study, wherein 50 women sero-positive for ANA served as study group (ANA+ group) and 50 women seronegative for ANA served as control group (ANA- group), and patients were age-matched in the two groups. ANA level in serum and FF were examined by ELISA, and immunofluorescence assay was employed to detect IgG within embryos.
In ANA+ group, 36 women showed positive ANA both in serum and FF on the day of ovum pick-up (OPU), wherein 34 exhibited fluorescence of IgG in embryos. All the 50 women in control group showed negative results in serum and FF ANA on OPU day and in embryo immunofluorescence. Serum ANA level positively correlated with FF ANA level. IVF outcomes were significantly poorer in ANA+ group, mainly reflecting the less number of high-quality embryos, reduced rates of pregnancy, clinical pregnancy, and implantation.
These data demonstrated the existence of ANA in FF and embryos, which may exert detrimental impact on IVF outcome.
检测卵泡液（FF）和胚胎中是否存在抗核抗体（ANA），以评估其对体外受精（IVF）结局的潜在影响。
本研究共纳入 100 名接受 IVF/ICSI 治疗的女性，其中 50 名抗核抗体阳性的女性作为研究组（ANA+组），50 名抗核抗体阴性的女性作为对照组（ANA-组）。两组患者年龄匹配。采用 ELISA 法检测血清和 FF 中的 ANA 水平，免疫荧光法检测胚胎中的 IgG。
在 ANA+组中，36 名女性在取卵日（OPU）时血清和 FF 中均出现 ANA 阳性，其中 34 名女性胚胎中出现 IgG 荧光。对照组所有 50 名女性在 OPU 日血清和 FF ANA 以及胚胎免疫荧光中均为阴性。血清 ANA 水平与 FF ANA 水平呈正相关。ANA+组的 IVF 结局明显较差，主要表现为优质胚胎数量减少、妊娠率、临床妊娠率和种植率降低。
这些数据表明 ANA 存在于 FF 和胚胎中，可能对 IVF 结局产生不良影响。
