Drexel University, Department of Chemical and Biological Engineering, Philadelphia, PA 19104, USA +1 215 895 2236 ; +1 215 895 5837 ;
Expert Opin Drug Discov. 2009 Dec;4(12):1237-51. doi: 10.1517/17460440903386643.
There is a growing need for inexpensive and highly sensitive methods in high-throughput format for measuring drug-target interactions. Cantilever biosensors are ultra-high sensitive electromechanical sensors that have been successfully used for label-free detection of a large number of biological entities. They are emerging as a technology that appears attractive for high-throughput drug discovery applications. Therefore, a brief description of this technology is provided here.
The objective of this review is to present an overview of the development in cantilever biosensor field reported to date and examine applications of cantilever biosensors for biomolecular interaction characterization and drug discovery. The subareas included in the review are: cantilever measurement principles, various interactions measured such as DNA-DNA, protein-protein, membrane protein-ligand and DNA-protein. Discussion on biomarker detection is also included.
We analyze the recent publications on cantilever biosensors with an emphasis on drug discovery and biomarker detection. The reader will become informed on the detection sensitivity and limit of detection achieved to date. A description of ongoing commercial activity that is likely to result in a practical instrument for researchers is also included. Technical issues that concern current researchers in the field of cantilever biosensors are also summarized.
The cantilever biosensors provide an extraordinarily sensitive method for binding affinity measurement using label-free reagents. They can be fabricated in array format for high-throughput applications using semiconductor manufacturing techniques. When they become commercially available in future, it is anticipated that they will be cost-competitive and easy to use in a research bioanalytical laboratory.
对于以高通量格式测量药物-靶标相互作用的廉价且高灵敏度的方法的需求日益增长。悬臂式生物传感器是超高灵敏度的机电传感器，已成功用于对大量生物实体进行无标记检测。它们作为一种似乎对高通量药物发现应用具有吸引力的技术而出现。因此，这里提供了对此技术的简要描述。
本综述的目的是对迄今为止报道的悬臂式生物传感器领域的发展进行概述，并检查悬臂式生物传感器在生物分子相互作用表征和药物发现中的应用。综述中包括的子领域是：悬臂式测量原理、测量的各种相互作用，如 DNA-DNA、蛋白质-蛋白质、膜蛋白-配体和 DNA-蛋白质。还包括对生物标志物检测的讨论。
我们分析了最近关于悬臂式生物传感器的出版物，重点是药物发现和生物标志物检测。读者将了解到迄今为止实现的检测灵敏度和检测限。还包括正在进行的商业活动的描述，这些活动很可能会为研究人员带来实用的仪器。还总结了悬臂式生物传感器领域当前研究人员关注的技术问题。
悬臂式生物传感器提供了一种使用无标记试剂进行结合亲和力测量的极其灵敏的方法。它们可以使用半导体制造技术以阵列格式制造，用于高通量应用。当它们在未来商业化时，预计它们将具有成本竞争力，并且易于在研究生物分析实验室中使用。
