高分辨率熔解曲线分析用于检测中国健康学童携带的两种耐红霉素百日咳博德特氏菌菌株。
High-resolution melting analysis for the detection of two erythromycin-resistant Bordetella pertussis strains carried by healthy schoolchildren in China.
机构信息
National Centre for Chronic and Non-communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, China.
出版信息
Clin Microbiol Infect. 2013 Jun;19(6):E260-2. doi: 10.1111/1469-0691.12161. Epub 2013 Mar 11.
Two erythromycin-resistant strains of Bordetella pertussis were isolated from nasopharyngeal specimens of two asymptomatic schoolchildren in China. High-resolution melting and sequencing analyses confirmed the homogeneous A2047G mutation in 23S rRNA genes of the two isolates. High-resolution melting (HRM) analysis is a useful assay for the rapid detection of erythromycin-resistant B. pertussis. The appearance of erythromycin-resistant B. pertussis strains in China is alarming.
两株耐红霉素的百日咳博德特氏菌从中国两名无症状学龄儿童的鼻咽标本中分离得到。高分辨率熔解和测序分析证实了两株分离株 23S rRNA 基因中的同质 A2047G 突变。高分辨率熔解（HRM）分析是一种快速检测耐红霉素百日咳博德特氏菌的有用方法。耐红霉素百日咳博德特氏菌菌株在中国的出现令人震惊。
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