Jensen Uffe B, Ghazizadeh Soosan, Owens David M
Department of Clinical Genetics, Aarhus University Hospital, Aarhus, Denmark.
Methods Mol Biol. 2013;989:61-9. doi: 10.1007/978-1-62703-330-5_6.
During homeostasis, adult mammalian skin turnover is maintained by a number of multipotent and -unipotent epithelial progenitors located either in the epidermis, hair follicle, or sebaceous gland. Recent work has illustrated that these various progenitor populations reside in regionalized niches and are phenotypically distinct from one another. This degree of heterogeneity within the progenitor cell landscape in the cutaneous epithelium complicates our ability to target, purify, and manipulate cutaneous epithelial stem cell subpopulations in adult skin. The techniques outlined in this chapter describe basic procedures for the isolation and purification of murine epithelial progenitors and assessing their capacity for ex vivo propagation.
在稳态过程中，成年哺乳动物皮肤的更新由位于表皮、毛囊或皮脂腺中的多种多能和单能上皮祖细胞维持。最近的研究表明，这些不同的祖细胞群体位于区域化的微环境中，并且在表型上彼此不同。皮肤上皮中祖细胞景观的这种异质性程度使我们在成年皮肤中靶向、纯化和操纵皮肤上皮干细胞亚群的能力变得复杂。本章概述的技术描述了分离和纯化小鼠上皮祖细胞并评估其体外增殖能力的基本程序。
