Behnia Ali, Haghighat Abbas, Talebi Ardeshir, Nourbakhsh Nosrat, Heidari Fariba
Ali Behnia, Member of Students' Research Center, Isfahan University of Medical Sciences, 81745-319 Isfahan, Iran.
World J Stem Cells. 2014 Sep 26;6(4):505-10. doi: 10.4252/wjsc.v6.i4.505.
To investigate the effect of stem cells from human exfoliated deciduous teeth (SHED) transplanted for bone regeneration in the dog mandibular defect.
In this prospective comparative study, SHEDs had been isolated 5 years ago from human exfoliated deciduous teeth. The undifferentiated stem cells were seeded into mandibular bone through-and-through defects of 4 dogs. Similar defects in control group were filled with cell-free collagen scaffold. After 12 wk, biopsies were taken and morphometric analysis was performed. The percentage of new bone formation and foreign body reaction were measured in each case. The data were subject to statistical analysis using the Mann-Whitney U and Kruskalwalis statistical tests. Differences at P < 0.05 was considered as significant level.
There were no significant differences between control and SHED-seeded groups in connective tissue (P = 0.248), woven bone (P = 0.248) and compact bone (P = 0.082). There were not any side effects in transplanted SHED group such as teratoma or malignancy and abnormalities in this period.
SHEDs which had been isolated and characterized 5 years ago and stored with cryopreservation banking were capable of proliferation and osteogenesis after 5 years, and no immune response was observed after three months of seeded SHEDs.
研究人乳牙脱落干细胞（SHED）移植对犬下颌骨缺损骨再生的影响。
在这项前瞻性对照研究中，SHED于5年前从人脱落乳牙中分离得到。将未分化的干细胞植入4只犬下颌骨的贯通性缺损处。对照组的类似缺损用无细胞胶原支架填充。12周后，取组织活检并进行形态计量分析。测量每种情况下新骨形成的百分比和异物反应。数据采用Mann-Whitney U检验和Kruskal-Wallis统计检验进行统计学分析。P < 0.05的差异被认为具有显著意义。
对照组和植入SHED的组在结缔组织（P = 0.248）、编织骨（P = 0.248）和密质骨（P = 0.082）方面无显著差异。在这一时期，植入SHED的组没有出现任何副作用，如畸胎瘤、恶性肿瘤或异常情况。
5年前分离并鉴定、通过冷冻保存库储存的SHED在5年后仍具有增殖和成骨能力，植入SHED三个月后未观察到免疫反应。
