Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 Shogoin, Kawahara-Cho, Sakyo-Ku, Kyoto, Japan.
J Orthop Res. 2013 Jul;31(7):1026-31. doi: 10.1002/jor.22332. Epub 2013 Mar 11.
The aim of this study was to elucidate whether the levels of serum biomarkers reflect the progression of osteoarthritis (OA) induced by different levels of exercise. Thirty-five Wistar rats subjected to anterior cruciate ligament transaction (ACLT) were divided into three groups: Control, moderate running, and intense running. Twelve rats (moderate running without ACLT) were allocated as a naive group. Running was performed on a motorized treadmill, at a speed of 18 m/min for 30 min/day (moderate and naive) or 60 min/day (intense) for 3 days per week. After 2 or 4 weeks, OA histopathology in the knees was evaluated using the Osteoarthritis Research Society International (OARSI) score, and the serum levels of cleaved collagen type II (C2C) and procollagen II C-propeptide (CPII) were analyzed. The OARSI score deteriorated in the intense running group after 2 weeks and the serum C2C/CPII ratio suggested the development of OA. At 4 weeks, the C2C/CPII ratio suggested there would be deterioration in the OARSI score but the score did not differ significantly between the moderate and intense running groups. C2C/CPII ratio had 13-25% correlation with the OARSI histological score. Thus, in rat experimental OA, the OARSI score could be partially predicted by the C2C/CPII ratio as a serum biomarker of OA.
本研究旨在阐明血清生物标志物的水平是否反映了不同运动水平引起的骨关节炎（OA）的进展。35 只 Wistar 大鼠接受前交叉韧带横断术（ACLT）后分为三组：对照组、中度跑步组和剧烈跑步组。12 只大鼠（无 ACLT 的中度跑步）被分配为对照组。在电动跑步机上以 18 m/min 的速度进行跑步，每天 30 分钟（中度和对照组）或 60 分钟（剧烈），每周 3 天。2 或 4 周后，使用骨关节炎研究协会国际评分（OARSI）评估膝关节的 OA 组织病理学，并分析血清中 II 型胶原裂解产物（C2C）和 II 型前胶原 C 端肽（CPII）的水平。在 2 周后，剧烈跑步组的 OARSI 评分恶化，血清 C2C/CPII 比值提示 OA 发展。4 周时，C2C/CPII 比值提示 OARSI 评分会恶化，但中度和剧烈跑步组之间的评分无显著差异。C2C/CPII 比值与 OARSI 组织学评分有 13-25%的相关性。因此，在大鼠实验性 OA 中，OARSI 评分可以部分通过 C2C/CPII 比值预测，作为 OA 的血清生物标志物。
