Department of Otorhinolaryngology, Faculty of Medicine, Pontificia Universidad Católica de Chile, Marcoleta 350, 2°Piso, Santiago 8330033, Chile.
Biomed Res Int. 2013;2013:478713. doi: 10.1155/2013/478713. Epub 2013 Feb 5.
Mucociliary transport (MCT) is a defense mechanism of the airway. To study the underlying mechanisms of MCT, we have both developed an experimental model of cultures, from human adenoid tissue of ciliated and secretory cells, and characterized the response to local chemical signals that control ciliary activity and the secretion of respiratory mucins in vitro.
In ciliated cell cultures, ciliary beat frequency (CBF) and intracellular Ca(2+) levels were measured in response to ATP, UTP, and adenosine. In secretory cultures, mucin synthesis and secretion were identified by using immunodetection. Mucin content was taken from conditioned medium and analyzed in the presence or absence of UTP.
Enriched ciliated cell monolayers and secretory cells were obtained. Ciliated cells showed a basal CBF of 10.7 Hz that increased significantly after exposure to ATP, UTP, or adenosine. Mature secretory cells showed active secretion of granules containing different glycoproteins, including MUC5AC.
Culture of ciliated and secretory cells grown from adenoid epithelium is a reproducible and feasible experimental model, in which it is possible to observe ciliary and secretory activities, with a potential use as a model to understand mucociliary transport control mechanisms.
黏液纤毛传输（Mucociliary Transport，MCT）是气道的一种防御机制。为了研究 MCT 的潜在机制，我们已经开发了一种实验模型，用于培养人腺样体的纤毛和分泌细胞，并对体外控制纤毛活动和呼吸黏液分泌的局部化学信号的反应进行了特征描述。
在纤毛细胞培养物中，测量了细胞内 Ca²⁺水平和对 ATP、UTP 和腺苷的反应性。在分泌培养物中，通过免疫检测鉴定了粘蛋白的合成和分泌。从条件培养基中提取粘蛋白含量，并在存在或不存在 UTP 的情况下进行分析。
获得了富含纤毛细胞的单层和分泌细胞。纤毛细胞的基础 CBF 为 10.7 Hz，暴露于 ATP、UTP 或腺苷后显著增加。成熟的分泌细胞显示出含有不同糖蛋白（包括 MUC5AC）的颗粒的活跃分泌。
从腺样体上皮生长的纤毛和分泌细胞的培养是一种可重复且可行的实验模型，可观察到纤毛和分泌活动，可作为理解黏液纤毛传输控制机制的模型。
