Dygai A M, Skurikhin E G, Ermakova N N, Pershina O V, Krupin V A, Reztsova A M, Ermolaeva L A, Khmelevskaya E S, Artamonov A V, Bekarev A A, Madonov P G, Kinsht D N
Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk, Russia.
Bull Exp Biol Med. 2013 Jan;154(3):388-92. doi: 10.1007/s10517-013-1957-z.
Hyaluronidase immobilized on polyethylenoxide obtained by electron bean synthesis was administered intranasally and intravenously to C57Bl/6 mice after intratracheal bleomycin and the enzyme effects on the development of pneumofibrosis in animals were studied. Intranasal immobilized hyaluronidase prevented connective tissue growth in the lungs exposed to bleomycin and virtually did not modulate the infiltration of the alveolar and alveolar duct interstitium by inflammatory cells (lymphocytes, macrophages, neutrophils, plasma cells). The antifibrotic effect developed sooner after intranasal inoculation of immobilized hyaluronidase and was more pronounced than after intranasal native hyaluronidase. Intravenous injection of immobilized hyaluronidase did not modify the inflammatory process and deposition of collagen fibrils in the lung parenchyma in pneumofibrosis.
将通过电子束合成获得的固定在聚环氧乙烷上的透明质酸酶在气管内注射博来霉素后经鼻内和静脉内给予C57Bl/6小鼠，并研究该酶对动物肺纤维化发展的影响。鼻内固定化透明质酸酶可防止暴露于博来霉素的肺部结缔组织生长，并且实际上不会调节炎症细胞（淋巴细胞、巨噬细胞、中性粒细胞、浆细胞）对肺泡和肺泡管间质的浸润。鼻内接种固定化透明质酸酶后抗纤维化作用出现得更快，且比鼻内注射天然透明质酸酶后更明显。静脉注射固定化透明质酸酶不会改变肺纤维化时肺实质中的炎症过程和胶原纤维沉积。
