司哌隆与固定化透明质酸酶联合应用对部分可逆性和不可逆性中毒性肺纤维化的抗炎和抗纤维化作用

Anti-inflammatory and antifibrotic effects of a combination of spiperone and immobilized hyaluronidase on partially reversible and irreversible toxic pneumofibrosis.

作者信息

Skurikhin E G, Ermakova N N, Reztsova A M, Pershina O V, Khmelevskaya E S, Krupin V A, Stepanova I E, Reztsova V M, Reihard D V, Artamonov A V, Bekarev A A, Madonov P G, Kinsht D N, Goldberg V E, Dygai A M

机构信息

Research Institute of Pharmacology, Siberian Division of the Russian Academy of Medical Sciences, Tomsk; Scientific Future Management, Novosibirsk, Russia.

出版信息

Bull Exp Biol Med. 2013 Nov;156(1):53-8. doi: 10.1007/s10517-013-2276-0.

DOI:10.1007/s10517-013-2276-0

PMID:24319728

Abstract

The possibility of boosting antifibrotic activity of testicular hyaluronidase immobilized on polyethylene oxide with spiperone was studied on the bleomycin models of a single (partially reversible pneumofibrosis) and repeated (irreversible pneumofibrosis) injuries to the alveolar epithelium in C57Bl/6 mice. The antifibrotic effect was more pronounced after successive treatment with immobilized hyaluronidase and spiperone than after individual treatment with each of the compounds: no collagen deposition in the parenchyma of bleomycin-damaged lungs was found. The decrease in inflammatory cell (lymphocytes, macrophages, neutrophils, plasma cells) infiltration of the alveoli and alveolar tracts interstitium in mice treated by immobilized hyaluronidase and spiperone did not differ from the anti-inflammatory effect of spiperone monotherapy.

摘要

在C57Bl/6小鼠肺泡上皮单次（部分可逆性肺纤维化）和反复（不可逆性肺纤维化）博来霉素损伤模型上，研究了用螺哌隆增强固定在聚环氧乙烷上的睾丸透明质酸酶抗纤维化活性的可能性。与单独使用每种化合物相比，固定化透明质酸酶和螺哌隆连续治疗后的抗纤维化作用更明显：在博来霉素损伤的肺实质中未发现胶原沉积。固定化透明质酸酶和螺哌隆处理的小鼠肺泡和肺泡道间质中炎症细胞（淋巴细胞、巨噬细胞、中性粒细胞、浆细胞）浸润的减少与螺哌隆单药治疗的抗炎效果无差异。