Onuki Tomoyo, Nihonyanagi Shin, Nakamura Masaki, Ide Toshimitsu, Hattori Jun, Kanoh Yuhsaku, Soma Kazui
Department of Clinical Laboratory, Kitasato University Hospital.
Kansenshogaku Zasshi. 2013 Jan;87(1):33-8. doi: 10.11150/kansenshogakuzasshi.87.33.
Clostridium tetani is widely distributed in ground or mud, especially in field and pond-shore surface layers. C. tetani is rarely isolated from specimens of patients with tetanus, and is generally diagnosed based on clinical symptoms such as trismus or general tonic spasms. This means that positive C. tetani infection is rarely diagnosed bacterially. Using gram straing, we identified C. tetani in specimens from patients suspected of C. tetani infection brought to the Kitasato University Hospital emergency center. Rapid gram staining information in the bacteriology laboratory is expected to improve recovery from C. tetani infection. It is therefore necessary to ensure clinical specimen quality control, and to keep standard strains of rare bacteria for isolation and identification.
破伤风梭菌广泛分布于土壤或淤泥中，尤其是在田野和池塘岸边的表层。破伤风梭菌很少从破伤风患者的标本中分离出来，一般根据牙关紧闭或全身强直性痉挛等临床症状进行诊断。这意味着破伤风梭菌感染阳性很少通过细菌学诊断。我们使用革兰氏染色法，在送往北里大学医院急诊中心的疑似破伤风梭菌感染患者的标本中鉴定出了破伤风梭菌。预计细菌学实验室的快速革兰氏染色信息将有助于提高破伤风梭菌感染的治愈率。因此，有必要确保临床标本的质量控制，并保存稀有细菌的标准菌株用于分离和鉴定。
