Guffey J Stephen, Payne William, Jones Tyler, Martin Kyle
Department of Physical Therapy, Arkansas State University, State University, ArkaAR 72467, USA.
Photomed Laser Surg. 2013 Apr;31(4):179-82. doi: 10.1089/pho.2012.3450. Epub 2013 Mar 13.
The aim of this study was to determine whether Staphylococcus aureus could develop a resistance to 405 nm light.
Research indicates that certain wavelengths and treatment parameters of light promote growth of bacteria; however, our earlier work indicated that blue light effectively inactivates growth of S. aureus in vitro.
S. aureus was tested because of its frequent isolation from skin infections and wounds. The organism was treated with 405 nm light from supraluminous diodes at a dose of 9 J/cm(2).
As anticipated, blue light produced a significant (p≤0.05) inactivation of S. aureus growth. Subsequent applications of blue light to subcultured generations of S. aureus were increasingly effective through four stages (generations). Beginning with stage five, a decrease in effectiveness (resistance) was observed.
Appropriate doses of 405-nm blue light inhibit the growth of S. aureus in vitro. This research does suggest that S. aureus may be capable of developing resistance to blue light irradiation.
本研究旨在确定金黄色葡萄球菌是否会对405纳米的光产生抗性。
研究表明，特定波长和治疗参数的光会促进细菌生长；然而，我们早期的研究表明，蓝光能在体外有效抑制金黄色葡萄球菌的生长。
对金黄色葡萄球菌进行测试是因为它经常从皮肤感染和伤口中分离出来。用来自超发光二极管的405纳米光以9焦耳/平方厘米的剂量处理该微生物。
正如预期的那样，蓝光使金黄色葡萄球菌的生长显著（p≤0.05）失活。随后对传代培养的金黄色葡萄球菌进行蓝光处理，经过四个阶段（代），效果越来越显著。从第五阶段开始，观察到效果（抗性）有所下降。
适当剂量的405纳米蓝光在体外抑制金黄色葡萄球菌的生长。这项研究确实表明金黄色葡萄球菌可能会对蓝光照射产生抗性。
