Department of Surgery, Weill Cornell Medical College, New York, NY 10065, USA.
Development. 2013 Apr;140(8):1639-44. doi: 10.1242/dev.093260. Epub 2013 Mar 13.
The GATA4 transcription factor is implicated in promoting cardiogenesis in combination with other factors, including TBX5, MEF2C and BAF60C. However, when expressed in embryonic stem cells (ESCs), GATA4 was shown to promote endoderm, not cardiac mesoderm. The capacity of related GATA factors to promote cardiogenesis is untested. We found that expression of the highly related gene, Gata5, very efficiently promotes cardiomyocyte fate from murine ESCs. Gata5 directs development of beating sheets of cells that express cardiac troponin T and show a full range of action potential morphologies that are responsive to pharmacological stimulation. We discovered that by removing serum from the culture conditions, GATA4 and GATA6 are each also able to efficiently promote cardiogenesis in ESC derivatives, with some distinctions. Thus, GATA factors can function in ESC derivatives upstream of other cardiac transcription factors to direct the efficient generation of cardiomyocytes.
GATA4 转录因子与其他因子（包括 TBX5、MEF2C 和 BAF60C）结合，被认为能促进心脏发生。然而，在胚胎干细胞（ESCs）中表达时，GATA4 被证明能促进内胚层，而不是心脏中胚层的发生。相关 GATA 因子促进心脏发生的能力尚未得到测试。我们发现，高度相关的基因 Gata5 的表达非常有效地促进了来自小鼠 ESCs 的心肌细胞命运。Gata5 指导形成表达心肌肌钙蛋白 T 的搏动细胞片层，并表现出对药物刺激有反应的全范围动作电位形态。我们发现，通过从培养条件中去除血清，GATA4 和 GATA6 各自也能够有效地促进 ESC 衍生物中的心脏发生，存在一些区别。因此，GATA 因子可以在 ESC 衍生物中的其他心脏转录因子之前发挥作用，以指导心肌细胞的有效生成。
