Cardiovascular Center & Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam 13620, Korea.
Molecular Medicine & Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 03080, Korea.
BMB Rep. 2018 Feb;51(2):85-91. doi: 10.5483/bmbrep.2018.51.2.176.
Pluripotent stem cell (PSC) variations can cause significant differences in the efficiency of cardiac differentiation. This process is unpredictable, as there is not an adequate indicator at the undifferentiated stage of the PSCs. We compared global gene expression profiles of two PSCs showing significant differences in cardiac differentiation potential. We identified 12 up-regulated genes related to heart development, and we found that 4 genes interacted with multiple genes. Among these genes, Gata6 is the only gene that was significantly induced at the early stage of differentiation of PSCs to cardiomyocytes. Gata6 knock-down in PSCs decreased the efficiency of cardiomyocyte production. In addition, we analyzed 6 mESC lines and 3 iPSC lines and confirmed that a positive correlation exists between Gata6 levels and efficiency of differentiation into cardiomyocytes. In conclusion, Gata6 could be utilized as a biomarker to select the best PSC lines to produce PSC-derived cardiomyocytes for therapeutic purposes. [BMB Reports 2018; 51(2): 85-91].
多能干细胞（PSC）的变化可能导致心脏分化效率的显著差异。这个过程是不可预测的，因为在 PSC 的未分化阶段没有足够的指标。我们比较了两种具有显著心脏分化潜力差异的 PSC 的全基因表达谱。我们鉴定出了 12 个与心脏发育相关的上调基因，并且发现其中 4 个基因与多个基因相互作用。在这些基因中，Gata6 是 PSC 向心肌细胞早期分化时唯一显著诱导的基因。在 PSC 中敲低 Gata6 会降低心肌细胞产生的效率。此外，我们分析了 6 条 mESC 系和 3 条 iPSC 系，并证实 Gata6 水平与分化为心肌细胞的效率之间存在正相关关系。总之，Gata6 可用作生物标志物，以选择最佳的 PSC 系来产生用于治疗目的的 PSC 衍生的心肌细胞。[BMB 报告 2018；51(2):85-91]。
