State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, PR China.
J Food Sci. 2013 Apr;78(4):M587-93. doi: 10.1111/1750-3841.12079. Epub 2013 Mar 12.
Enterococcus faecium YF5, a strain previously isolated from sourdough, was assessed for safety and probiotic potential. Its virulence and antibiotic resistant phenotypes (cytolysin and gelatinase production, antibiotic susceptibility) and genes (cylA, gelE, ace, agg, esp, and vanA) were surveyed. Results indicated that the tested virulence determinants were nontoxic. In addition, E. faecium YF5 was sensitive to 3 antibiotics such as amoxicillin, vancomycin, and chloramphenicol. Furthermore, results of in vivo animal acute oral toxicity of E. faecium YF5 studies were similar to the control group that indicated no abnormalities. In addition, E. faecium YF5 stably survived in low pH, bile salts, gastric, and intestinal fluids in vitro. Moreover, E. faecium YF5 was found to adhere to human colon cancer cell line HT-29 at 3.39 (±0.67) × 10(5) CFU/mL. When cocultured with pathogenic organisms (Enterobacter sakazakii CMCC45402, Escherichia coli CMCC44102, enterohemorrhage Escherichia coli O157: H7 CMCC44828, Salmonella Typhimurium CMCC50071, Shigella flexneri 301, and Shigella sonnei ATCC 29930) and 2 gram-positive strains (Listeria monocytogenes CMCC54001 and Staphylococcus aureus CMCC 26003), it inhibited these foodborne pathogens with exception of S. aureus. Therefore, E. faecium YF5 can be regarded as a safe strain and it may be used as a probiotic preparation or for microecologics.
屎肠球菌 YF5 是一株从酸面团中分离到的益生菌，对其进行了安全性和益生菌潜力评估。检测了其毒力表型（细胞溶解素和明胶酶的产生、抗生素敏感性）和基因（ cylA 、 gelE 、 ace 、 agg 、 esp 和 vanA ）。结果表明，所测试的毒力决定因素无毒性。此外，屎肠球菌 YF5 对 3 种抗生素（阿莫西林、万古霉素和氯霉素）敏感。此外，屎肠球菌 YF5 体内动物急性口服毒性研究的结果与对照组相似，表明无异常。此外，屎肠球菌 YF5 能够在体外低 pH 值、胆盐、胃和肠液中稳定存活。此外，屎肠球菌 YF5 被发现能够以 3.39（±0.67）×10(5)CFU/mL 的浓度黏附于人结肠癌细胞系 HT-29。当与病原菌（阪崎肠杆菌 CMCC45402 、大肠埃希菌 CMCC44102 、产肠出血性大肠埃希菌 O157：H7 CMCC44828 、鼠伤寒沙门氏菌 CMCC50071 、福氏志贺菌 301 和宋内志贺菌 ATCC 29930 ）和 2 株革兰氏阳性菌（单核细胞增生李斯特菌 CMCC54001 和金黄色葡萄球菌 CMCC 26003 ）共培养时，除了金黄色葡萄球菌之外，它都能抑制这些食源性病原菌。因此，屎肠球菌 YF5 可被视为一种安全菌株，可用于益生菌制剂或微生态制剂。
