School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Key Laboratory of Biomass Energy and Environmental Biotechnology, Yunnan Normal University, Kunming, 650092, Yunnan Province, China.
Plant Cell Environ. 2013 Aug;36(8):1564-72. doi: 10.1111/pce.12092. Epub 2013 Apr 8.
Nitric oxide (NO) is a second messenger with multifunction that is involved in plant growth, development and the acquisition of stress tolerance. In recent years, hydrogen sulphide (H(2)S) has been found to have similar functions, but crosstalk between NO and H(2)S in the acquisition of heat tolerance is not clear. In this study, pretreatment with the NO donor sodium nitroprusside (SNP) improved the survival percentage of maize seedlings and alleviated an increase in electrolyte leakage and a decrease in tissue vitality as well as accumulation of malondialdehyde, indicating that pretreatment with SNP improved the heat tolerance of maize seedlings. In addition, pretreatment with SNP enhanced the activity of L-cystine desulfhydrase, which, in turn, induced accumulation of endogenous H(2)S, while application of H(2)S donors, NaHS and GYY4137, increased endogenous H(2)S content, followed by mitigating increase in electrolyte leakage and enhanced survival percentage of seedlings under heat stress. Interestingly, SNP-induced heat tolerance was enhanced by application of NaHS and GYY4137, but was eliminated by inhibitors of H(2)S synthesis DL-propargylglycine, aminooxyacetic acid, potassium pyruvate and hydroxylamine, and the H(2)S scavenger hypotaurine. All of the above-mentioned results suggest that SNP pretreatment could improve heat tolerance, and H(2)S may be a downstream signal molecule in NO-induced heat tolerance of maize seedlings.
一氧化氮（NO）是一种具有多功能的第二信使，参与植物的生长、发育和获得胁迫耐受性。近年来，发现硫化氢（H₂S）也具有类似的功能，但 NO 和 H₂S 在获得耐热性方面的串扰尚不清楚。本研究中，用一氧化氮供体硝普钠（SNP）预处理提高了玉米幼苗的存活率，并缓解了电解质泄漏的增加和组织活力的降低以及丙二醛的积累，表明 SNP 预处理提高了玉米幼苗的耐热性。此外，SNP 预处理增强了 L-胱氨酸脱硫酶的活性，进而诱导内源性 H₂S 的积累，而 H₂S 供体 NaHS 和 GYY4137 的应用增加了内源性 H₂S 的含量，随后缓解了热应激下电解质泄漏的增加和幼苗存活率的提高。有趣的是，SNP 诱导的耐热性通过应用 NaHS 和 GYY4137 得到增强，但通过 H₂S 合成抑制剂 DL-丙炔甘氨酸、氨基氧乙酸、丙酮酸钾和羟胺以及 H₂S 清除剂硫代牛磺酸消除，所有这些结果表明 SNP 预处理可以提高耐热性，并且 H₂S 可能是 NO 诱导玉米幼苗耐热性的下游信号分子。
