University of Belgrade, School of Medicine, Belgrade, Serbia.
J Biol Regul Homeost Agents. 2013 Jan-Mar;27(1):35-44.
The experiment compared the physiological function (insulin secretory capacity) and membrane integrity of human adult pancreatic islets incubated in culture at 37°C and 24°C. Pancreatic tissue was digested with Collagenase XI, using a non-automated method. Cultures were incubated at 37°C and 24°C. Secretory capacity of the islets is determined by measuring of the stimulation index (SI) on the 1st, 3rd and 7th day of cultivation. Membrane integrity of the islets was determined by dithizone staining. Both groups of examined cultures show a slight increase in SI during the incubation. However islets incubated at 24°C show higher SI values than those incubated at 37°C on the 1st, 3rd and 7th day of incubation. And on the first day of incubation, this difference was statistically significant (p <0.05). Islets incubated at 37°C showed preservation of membrane integrity, the islets are regular spherical shape, while those incubated at 24°C lose such an organization. During the seven-day cultivation, islets incubated at a standard temperature of 37°C show less preserve physiological functions in relation to cultures incubated at 24°C, but islets incubated at 37°C show more regular morphological forms.
该实验比较了在 37°C 和 24°C 下培养的人成年胰腺胰岛的生理功能（胰岛素分泌能力）和膜完整性。使用非自动化方法用胶原酶 XI 消化胰腺组织。在 37°C 和 24°C 下孵育培养物。在培养的第 1、3 和 7 天通过测量刺激指数 (SI) 来确定胰岛的分泌能力。胰岛的膜完整性通过二硫嗪染色来确定。两组检查培养物在孵育过程中均显示 SI 略有增加。然而，与在 37°C 孵育的胰岛相比，在 24°C 孵育的胰岛在孵育的第 1、3 和 7 天具有更高的 SI 值。并且在第 1 天的孵育中，这种差异具有统计学意义（p <0.05）。在 37°C 孵育的胰岛保持膜完整性，胰岛呈规则的球形，而在 24°C 孵育的胰岛则失去这种组织。在 7 天的培养过程中，与在 24°C 孵育的培养物相比，在标准温度 37°C 下孵育的胰岛在生理功能方面的保存较少，但在 37°C 下孵育的胰岛具有更规则的形态形式。
